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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGBW-m4046493
 
Resource Report
Resource Website
RRID:Addgene_145533 SARS-CoV-2 E (envelope) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724392.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046412
 
Resource Report
Resource Website
RRID:Addgene_145497 SARS-CoV-2 ORF7b Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725318.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:56 0
pGBW-m4046218
 
Resource Report
Resource Website
RRID:Addgene_145498 SARS-CoV-2 nsp2 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725298.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:56 0
pGBW-m4046479
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_145495 SARS-CoV-2 nsp4 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725300.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:56 1
pGBW-m4046468
 
Resource Report
Resource Website
RRID:Addgene_145496 SARS-CoV-2 nsp9 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725305.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046445
 
Resource Report
Resource Website
RRID:Addgene_145493 SARS-CoV-2 nsp9 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725305.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046424
 
Resource Report
Resource Website
RRID:Addgene_145538 SARS-CoV-2 endoRNAse Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725310.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046175
 
Resource Report
Resource Website
RRID:Addgene_145547 SARS-CoV-2 ORF7b Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725318.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046212
 
Resource Report
Resource Website
RRID:Addgene_145548 SARS-CoV-2 ORF7b Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725318.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046367
 
Resource Report
Resource Website
RRID:Addgene_145545 SARS-CoV-2 endoRNAse Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725310.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046340
 
Resource Report
Resource Website
RRID:Addgene_145543 SARS-CoV-2 leader protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725297.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046333
 
Resource Report
Resource Website
RRID:Addgene_145544 SARS-CoV-2 ORF3a protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724391.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046371
 
Resource Report
Resource Website
RRID:Addgene_145541 SARS-CoV-2 3C-like proteinase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725301.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046215
 
Resource Report
Resource Website
RRID:Addgene_145542 SARS-CoV-2 leader protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725297.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pGBW-m4046165
 
Resource Report
Resource Website
RRID:Addgene_145540 SARS-CoV-2 nsp7 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725303.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:57 0
pGBW-m4046324
 
Resource Report
Resource Website
RRID:Addgene_145549 SARS-CoV-2 ORF10 protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725255.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:57 0
pCAG-FlpeERT2
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_14756 Flpe-ERT2 fusion protein Saccharomyces cerevisiae Ampicillin PMID:17209010 Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation. Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin 2026-08-15 01:07:07 3
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)
 
Resource Report
Resource Website
RRID:Addgene_14841 PHO5 promoter UASm1 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin UASm1 (AAGCTT was substituted for CACGTT). Uninducible promoter. See Steger DJ et al Science 299(5603)114-116. 2026-08-15 01:07:09 0
pnEA-NpM-Scp20_18-49-GB1_AF
 
Resource Report
Resource Website
RRID:Addgene_148720 Scp20_18-49 Saccharomyces cerevisiae Ampicillin PMID:30053226 Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:10 0
pJRL2 GAL1pr VYFP (EB#1571)
 
Resource Report
Resource Website
RRID:Addgene_14847 GAL1 promoter Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:07:09 0

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