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Species: Homo sapiens
Genetic Insert: human KCNB1
Vector Backbone Description: Vector Backbone:pHA-CIneo (Promega’s pCI-neo vector modified with a HA-tag); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31682765
Proper citation: RRID:Addgene_124490 Copy
Species: Homo sapiens
Genetic Insert: PSPC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31844057
Comments: For wildtype PSPC1 see https://www.addgene.org/101764/
Proper citation: RRID:Addgene_124373 Copy
Species: Homo sapiens
Genetic Insert: Gal4-TET1-878-911 wildtype
Vector Backbone Description: Backbone Marker:University of Leicester; Vector Backbone:pLeicester 84; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279502
Proper citation: RRID:Addgene_124400 Copy
Species: Homo sapiens
Genetic Insert: human KCNB1
Vector Backbone Description: Backbone Marker:promega; Vector Backbone:pHA-CIneo (Promega’s pCI-neo vector modified with a HA-tag); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31682765
Proper citation: RRID:Addgene_124487 Copy
Species: Homo sapiens
Genetic Insert: Gal4-TET1-878-911(I894A mutation)
Vector Backbone Description: Backbone Marker:University of Leicester; Vector Backbone:pLeicester 84; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279502
Proper citation: RRID:Addgene_124404 Copy
Species: Homo sapiens
Genetic Insert: Gal4-TET1-878-911(T898A mutation)
Vector Backbone Description: Backbone Marker:University of Leicester; Vector Backbone:pLeicester 84; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279502
Proper citation: RRID:Addgene_124403 Copy
Species: Homo sapiens
Genetic Insert: GluR2_adRNA(1,20,6)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Comments: A second copy of the GluR2_adRNA_(1,20,6) is expressed from a mouse U6 promoter
Proper citation: RRID:Addgene_124619 Copy
Species: Homo sapiens
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124616 Copy
Species: Homo sapiens
Genetic Insert: NPM1 Homology Arms with linker-mTagRFP-T
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the C-terminus of human NPM1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: KPNSAVDGTAGPGSIAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_124608 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16352599
Proper citation: RRID:Addgene_12453 Copy
Species: Homo sapiens
Genetic Insert: EFP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16352599
Comments: Digestion by KpnI and XhoI should give a 5.4 kb fragment and a 2.4 kb fragment.
Compared to GenBank ID NP_005073.2, the EFP insert in this plasmid appears to contain a P385L mutation, whereas when compared to GenBank ID AB384815.1 the EFP insert appears to contain G89V and A185T mutations. These apparent mutations may represent valid polymorphisms and reflect the sequence of the original human cDNA clone. The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_12452 Copy
Species: Homo sapiens
Genetic Insert: WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: wild-type tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. Wild-type tRNA has high pol-III promoter activity with high processing. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124541 Copy
Species: Homo sapiens
Genetic Insert: delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124540 Copy
Species: Homo sapiens
Genetic Insert: deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124543 Copy
Species: Homo sapiens
Genetic Insert: delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124545 Copy
Species: Homo sapiens
Genetic Insert: deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124544 Copy
Species: Homo sapiens
Genetic Insert: deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124539 Copy
Species: Homo sapiens
Genetic Insert: c-Myc Renilla reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124525; Backbone Size:2326; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124532 Copy
Species: Homo sapiens
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124652 Copy
Species: Homo sapiens
Genetic Insert: P53 NLuc reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124526; Backbone Size:2328; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124533 Copy
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