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Species: Synthetic
Genetic Insert: TaCas9_H840A nickase
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91034 Copy
Species: Synthetic
Genetic Insert: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
Vector Backbone Description: Vector Backbone:pMOD_B2000; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91061 Copy
Species: Synthetic
Genetic Insert: dTaCas9-VPR
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91054 Copy
Species: Synthetic
Genetic Insert: AtCas9_H840A nickase
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91007 Copy
Species: Synthetic
Genetic Insert: AtCas9_H840A nickase
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91005 Copy
Species: Synthetic
Genetic Insert: AtCas9_D10A nickase
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91002 Copy
Species: Synthetic
Genetic Insert: HRP
Vector Backbone Description: Backbone Size:6142; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17961829
Proper citation: RRID:Addgene_44021 Copy
Species: Synthetic
Genetic Insert: GCaMP3
Vector Backbone Description: Backbone Marker:Addgene plasmid 43915; Backbone Size:9600; Vector Backbone:FU-MAP2-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22174877
Comments: The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915).
This destination vector was subsequently used to generate MAP2-GCaMP3 (GCaMP3 cloned from G-CaMP3, Addgene plasmid 22692).
The V5 and His tags present in the vector backbone are not expressed with the GCaMP3 insert.
Proper citation: RRID:Addgene_43917 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5072; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44277 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44279 Copy
Species: Synthetic
Genetic Insert: PAiRFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4064; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23842578
Proper citation: RRID:Addgene_44271 Copy
Species: Synthetic
Genetic Insert: QQR ZFN
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: QQR ZFN reference:
Even-Faitelson L, Samach A, Melamed-Bessudo C, Avivi-Ragolsky N, Levy AA (2011) Localized egg-cell expression of effector proteins for targeted modification of the Arabidopsis genome. The Plant Journal 68 (5):929-937. doi:10.1111/j.1365-313X.2011.04741.x
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44463 Copy
Species: Synthetic
Genetic Insert: AtCRU3 T494 TALENs
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: pZHY013 reference:
Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44464 Copy
Species: Synthetic
Genetic Insert: Horseradish peroxidase fused to the transmembrane domain of PDGFR
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23371551
Comments: This construct has the Ig k-chain leader sequence on the N-terminus, which directs the protein to the secretory pathway. HRP faces the extracellular side of the plasma membrane.
Proper citation: RRID:Addgene_44441 Copy
Species: Synthetic
Genetic Insert: PTETREG promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4456; Vector Backbone:pRS403; Vector Types:Yeast Expression, Synthetic Biology, Expression regulator/reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22511863
Comments: This plasmids was created as follows. First, the PTETREG promoter consisting of two tetO2 sites upstream of the minimal PCYC1 promoter was amplified from the pBB247 plasmid (Becskei, Séraphin, and Serrano, EMBO 2001, PMID: 11350942), and inserted into the pDN-G1GZmh plasmid between the AflII and BamHI sites instead of the PGAL1-D12 promoter resulting in the pDN-T2dGZmh plasmid. In order to facilitate the planned integration of the reporter plasmid into the his3Δ200 locus of the YPH500 strain, a small region bearing homology to the his3Δ200 locus was constructed by PCR and inserted in front of the HIS3 gene between the AhdI and AfeI sites of the pDN-T2dGZmh plasmid, resulting in the pDN-T2dGZmlh plasmid. Next, a second ADH1 terminator was removed from the pDN-T2dGZmlh plasmid, resulting in the final reporter pDN-T2dGZmxh plasmid bearing the yEGFP::zeoR fluorescent reporter gene under the control of the PTETREG rtTA-MF inducible promoter.
Proper citation: RRID:Addgene_44552 Copy
Species: Synthetic
Genetic Insert: Citrine-PTS1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10404; Vector Backbone:pMpGWB102; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23421791
Proper citation: RRID:Addgene_44592 Copy
Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: TAGCCA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
Proper citation: RRID:Addgene_44795 Copy
Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: GCTTAG
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
Proper citation: RRID:Addgene_44794 Copy
Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: TAACTT
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
Proper citation: RRID:Addgene_44796 Copy
Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: CAAATA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
Proper citation: RRID:Addgene_44790 Copy
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