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Species: Homo sapiens
Genetic Insert: FOXO Renilla reporter
Vector Backbone Description: Backbone Marker:Venken Lab, #124525; Backbone Size:2326; Vector Backbone:AlphaC Vector; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_124535 Copy
Species: Homo sapiens
Genetic Insert: deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124537 Copy
Species: Homo sapiens
Genetic Insert: RGECO-TnT
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7500; Vector Backbone:pShuttle CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30732532
Proper citation: RRID:Addgene_124643 Copy
Species: Homo sapiens
Genetic Insert: INPPL1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3 HIS C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11349134
Proper citation: RRID:Addgene_124645 Copy
Species: Homo sapiens
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:0; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16122702
Comments: EcoRV site was destroyed during cloning and replaced with a new NotI site.
Proper citation: RRID:Addgene_12461 Copy
Species: Homo sapiens
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16122702
Proper citation: RRID:Addgene_12460 Copy
Species: Homo sapiens
Genetic Insert: GluR2_adRNA(2,100,50)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Proper citation: RRID:Addgene_124622 Copy
Species: Homo sapiens
Genetic Insert: ADAR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Comments: Also expresses 2 copies of GluR2_adRNA(1,20,6) from human and mouse U6 promoters
Proper citation: RRID:Addgene_124620 Copy
Species: Homo sapiens
Genetic Insert: MCP_ADAR1 DD (E1008Q)_NLS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Comments: Also expresses MS2_adRNA targeting the RAB7A transcript
Proper citation: RRID:Addgene_124626 Copy
Species: Homo sapiens
Genetic Insert: MS2_adRNA_RAB7A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Proper citation: RRID:Addgene_124624 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving PD-L1 under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124684 Copy
Species: Homo sapiens
Genetic Insert: Metallothionein 1k
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTyb21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_105707 Copy
Species: Homo sapiens
Genetic Insert: Metallothionein 1h
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTyb21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_105706 Copy
Species: Homo sapiens
Genetic Insert: human carbonic anhydrase II thermostable variant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105668 Copy
Species: Homo sapiens
Genetic Insert: Metallothionein 1b
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTyb21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_105701 Copy
Species: Homo sapiens
Genetic Insert: Metallothionein 1e
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTyb21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32924499
Proper citation: RRID:Addgene_105702 Copy
Species: Homo sapiens
Genetic Insert: human carbonic anhydrase II thermostable variant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105666 Copy
Species: Homo sapiens
Genetic Insert: human carbonic anhydrase II thermostable variant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105667 Copy
Species: Homo sapiens
Genetic Insert: Champ1
Vector Backbone Description: Vector Backbone:pIRESneo2 GFP YTA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_105639 Copy
Species: Homo sapiens
Genetic Insert: HMGCR 3'UTR
Vector Backbone Description: Backbone Marker:Promega, psiCheck2; Vector Backbone:pDEST 765; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28102325
Comments: 3'UTR clones downstream of Renilla luciferase reporter gene
Proper citation: RRID:Addgene_105766 Copy
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