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Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2667; Vector Backbone:pDONR 221 P4r-P3r; Vector Types:Gateway MultiSite entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/12862 for additional plasmid map.
Proper citation: RRID:Addgene_32309 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32412 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32416 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 L5-L4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32417 Copy
Genetic Insert: oPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Comments: Gene Therapy (2006) 13, 641–645. doi:10.1038/sj.gt.3302698; published online 15 December 2005
'Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression. Schambach A, Bohne J, Baum C, Hermann FG, Egerer L, von Laer D, Giroglou T. Gene Ther. 2006 Apr;13(7):641-5.'
Addgene Sanger sequencing confirms that all four ATGs in the oPRE have been mutated to TAGs
Proper citation: RRID:Addgene_32414 Copy
Genetic Insert: ColE1 origin replication
Vector Backbone Description: Vector Backbone:pMS2Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11707328
Comments: This is a mycobacterial shuttle vector with a lower copy number in E. coli.
Proper citation: RRID:Addgene_32360 Copy
Species: Mus musculus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21669867
Comments: A W374C mutation is also present in the pEGFP-N1-CaMKKbeta constructs that were used in this publication.
Proper citation: RRID:Addgene_32453 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Schmidt-Dannert Lab; Backbone Size:4138; Vector Backbone:pBBRBB; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22033566
Proper citation: RRID:Addgene_32549 Copy
Species: Mus musculus
Genetic Insert: E1, ubiquitin-activating enzyme
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET-28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22012022
Proper citation: RRID:Addgene_32534 Copy
Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9620770
Proper citation: RRID:Addgene_32492 Copy
Species: Homo sapiens
Genetic Insert: HSF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4729; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12917326
Comments: Please note that mutation P448L in HSF1 was found during Addgene's quality control. This plasmid should function as reported in the associated publication.
Proper citation: RRID:Addgene_32538 Copy
Vector Backbone Description: Backbone Size:12000; Vector Backbone:pCAMBIA1301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20388670
Proper citation: RRID:Addgene_32470 Copy
Vector Backbone Description: Backbone Size:12000; Vector Backbone:pCAMBIA1301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20388670
Proper citation: RRID:Addgene_32471 Copy
Species: Mus musculus
Genetic Insert: CAMKIIalpha promoter 1.3kb version
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32577 Copy
Genetic Insert: Tcf/Lef:H2B-GFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21176145
Comments: To g ene r a t e t h e p T CF /L e f :H 2 B -GFP co n s t ru c t , s i x
copies of the TCF/Lef response elements together with
the hsp68 minimal promoter from the TCF/Lef-LacZ
reporter construct [11] were inserted into the AseI/Nhe1
sites of pCMV::H2B-GFP.
Proper citation: RRID:Addgene_32610 Copy
Species: Mus musculus
Genetic Insert: CAMKIIalpha promoter 0.4kb version
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32578 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2720; Vector Backbone:pENTR4; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21418658
Proper citation: RRID:Addgene_32686 Copy
Species: Mus musculus
Genetic Insert: Kir2.1-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32669 Copy
Genetic Insert: ECFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32594 Copy
Genetic Insert: IRES-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Comments: BMC Biotechnol. 2006 Jan 12;6:4.
Development of a new bicistronic retroviral vector with strong IRES activity.
Martin P, Albagli O, Poggi MC, Boulukos KE, Pognonec P.
Source
CNRS UMR6548, Parc Valrose, Université de Nice Sophia Antipolis, Nice, France. [email protected]
Proper citation: RRID:Addgene_32591 Copy
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