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Species: Homo sapiens
Genetic Insert: WAF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8242752
Comments: full-length WAF1 cDNA insert cloned into the NotI site of pCEP4.
Proper citation: RRID:Addgene_16450 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_451m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164452 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_453m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164453 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-mscRE4-minCMV-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
The Allen Institute for Brain Science recommends this plasmid as a potential substitute for Plasmid 163471: AiP1269-scAAV-mscRE4-minBGpromoter-SYFP2-WPRE3-bGHpA.
Proper citation: RRID:Addgene_164457 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_460m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164455 Copy
Genetic Insert: VSV-G
Vector Backbone Description: Vector Backbone:HDM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164440 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2144057
Comments: A 1.8kb XbaI fragment encoding wild-type p53 (from nucleotide -130 to 1671 relative to the ATG) was blunt-ended and ligated to BamHI linkers. This was then cloned into the unique BamHI site in the pCMV-Neo-Bam vector. This plasmid is also known as pC53-SN3.
Proper citation: RRID:Addgene_16434 Copy
Species: Synthetic
Genetic Insert: G-CEPIA1er
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8037; Vector Backbone:pLVX-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31346185
Proper citation: RRID:Addgene_164590 Copy
Species: Mus musculus
Genetic Insert: (Cyto)iGlucoSnFR-mRuby2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161775
Comments: The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (https://www.addgene.org/244084/) instead of this plasmid.
Please visit https://doi.org/10.1101/571422 for bioRxiv preprint.
Proper citation: RRID:Addgene_164514 Copy
Vector Backbone Description: Vector Backbone:PB-TAG-ERN; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33080218
Proper citation: RRID:Addgene_164519 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: oChIEF-tdtomato
Vector Backbone Description: Backbone Marker:Addgene plasmid 18917; Backbone Size:5079; Vector Backbone:AAV-FLEX-rev-ChR2-tdtomato; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21878344
Comments: Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function.
To minimize recombination, propagate this plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
For more information on growth conditions, please see www.addgene.org/18917 as this is the backbone vector. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence. Please see Reviews in the right column for an image of Addgene's digest with these enzymes.
Proper citation: RRID:Addgene_30541 Copy
Species: synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCSGFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19549299
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.
Proper citation: RRID:Addgene_30530 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518905
Proper citation: RRID:Addgene_30496 Copy
Species: Homo sapiens
Genetic Insert: p300
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30490 Copy
Species: Homo sapiens
Genetic Insert: BCL2-associated X protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:C3-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458670
Proper citation: RRID:Addgene_30533 Copy
Species: Bos taurus
Genetic Insert: Mature bovine mitochondrial elongation factor Tu
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8547323
Proper citation: RRID:Addgene_30535 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14675537
Proper citation: RRID:Addgene_30482 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14675537
Proper citation: RRID:Addgene_30484 Copy
Species: Homo sapiens
Genetic Insert: HDAC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14675537
Proper citation: RRID:Addgene_30483 Copy
Species: Homo sapiens
Genetic Insert: HDAC4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30486 Copy
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