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Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139118 Copy
Species: E. coli
Genetic Insert: tktBp-GFPuv
Vector Backbone Description: Vector Backbone:pSMART-HC Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32353228
Comments: DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid.
Proper citation: RRID:Addgene_139081 Copy
Species: E. coli
Genetic Insert: yibDp2-GFPuv
Vector Backbone Description: Vector Backbone:pSMART-HC Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32353228
Comments: DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid.
Proper citation: RRID:Addgene_139083 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139122 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139123 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139120 Copy
Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139126 Copy
Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139127 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139125 Copy
Species: Homo sapiens
Genetic Insert: iRFP:MTOR(2021-2113):[GGSA]2:GOLGB1(3097-3226)
Vector Backbone Description: Vector Backbone:piRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139313 Copy
Species: Mus musculus
Genetic Insert: mCherry:Mus musculus Prkd1(138-343)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139314 Copy
Species: Homo sapiens
Genetic Insert: ID1
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:p15A-PB; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Comments: Genomic sequence subcloned by recombineering from a clone of RPCI-11 human genomic DNA library. The exact DNA sequence in this plasmid differs somewhat from the reference human genome sequence because the reference sequence is a composite sequence, i.e., the reference ID1 region sequence may not be derived from the RPCI-11 BAC clone utilized for this construct.
The ID1 intron was placed after G198, i.e., after about a third of the sequence encoding the active site of the cre protein. See https://doi.org/10.1073/pnas.2011448117 and https://doi.org/10.1093%2Fnar%2Fgkq384. Thus, without splicing, no catalytically active cre protein sequence is expected to be translated from this sequence.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before using.
Proper citation: RRID:Addgene_139280 Copy
Species: Synthetic
Genetic Insert: cfmTurquoise
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:mTurquoise-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31020675
Proper citation: RRID:Addgene_139284 Copy
Species: Streptomyces achromogenes subsp. streptozoticus
Genetic Insert: sznE
Vector Backbone Description: Backbone Size:5308; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30728519
Proper citation: RRID:Addgene_139282 Copy
Species: Synthetic
Genetic Insert: Tus
Vector Backbone Description: Vector Backbone:pPD1060 Integration Vector (PuroR-P2A-DsRedExpress2); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Leonard Lab plasmid reference number: L1570
Proper citation: RRID:Addgene_139251 Copy
Species: Other
Genetic Insert: Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
Vector Backbone Description: Vector Backbone:pTagBFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139309 Copy
Genetic Insert: linker with pelB-leader, cloning site, flag-tag and p3
Vector Backbone Description: Vector Backbone:fADL-1e; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32872428
Proper citation: RRID:Addgene_139441 Copy
Species: Other
Genetic Insert: SNIPR_GFP
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating GFPmut3b with an ASV degradation tag
Proper citation: RRID:Addgene_139463 Copy
Species: Other
Genetic Insert: SNIPR_lacZ
Vector Backbone Description: Backbone Size:3324; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating a lacZ reporter protein
Proper citation: RRID:Addgene_139464 Copy
Species: Homo sapiens
Genetic Insert: TYK2 cDNA with 267nt of 5' UTR
Vector Backbone Description: Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11399767
Proper citation: RRID:Addgene_139354 Copy
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