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Genetic Insert: SP146/gp91/GP91/cHS4
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France),
the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive
site and was provided by Patrick Salmon (Department of Neuroscience,
Faculty of Medicine, University of Geneva, Switzerland).
Proper citation: RRID:Addgene_30480 Copy
Species: Homo sapiens
Genetic Insert: p300
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30489 Copy
Species: Mus musculus
Genetic Insert: macroH2A1.2-C
Vector Backbone Description: Vector Backbone:pDEST pCS2+ att-eGFP-HA (C term eGFP-HA); Vector Types:Mammalian Expression, Xenopus/Zebrafish expression.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552206
Proper citation: RRID:Addgene_30515 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22020504
Comments: In our experience, plasmid yields are highest when cells are harvest at late log stage.
Note that this plasmid also confers resistance to Spectinomycin, but Addgene provides it in an Amp stab.
Proper citation: RRID:Addgene_30505 Copy
Species: Mus musculus
Genetic Insert: Sin3A
Vector Backbone Description: Backbone Size:4354; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11571228
Proper citation: RRID:Addgene_30452 Copy
Species: Mus musculus
Genetic Insert: Sin3A
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pME18S; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30454 Copy
Genetic Insert: histone GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 12337; Backbone Size:8000; Vector Backbone:pCSC-SP-PW-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30456 Copy
Species: Xanthamonas oryzae
Genetic Insert: last NI repeat
Vector Backbone Description: Backbone Size:2460; Vector Backbone:pCS468; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31006 Copy
Species: Xanthamonas oryzae
Genetic Insert: last NG repeat
Vector Backbone Description: Backbone Size:2460; Vector Backbone:pCS468; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30995 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NG3
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30987 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NG2
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30986 Copy
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31021 Copy
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31018 Copy
Genetic Insert: GFP(FRT)lacZ
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11376165
Comments: FLP reporter: from green protein fluorescence to lacZ
Proper citation: RRID:Addgene_31124 Copy
Species: Homo sapiens
Genetic Insert: euchromatic histone-lysine N-methyltransferase 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6963; Vector Backbone:pLenti6/V5-D-TOPO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Spektor TM, Rice JC. Identification and characterization of posttranslational modification-specific binding proteins in vivo by mammalian tethered catalysis. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14808-13. Epub 2009 Aug 18.
Proper citation: RRID:Addgene_31113 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17214554
Comments: for Flp mediated site directed integration, tetracycline inducibility
Proper citation: RRID:Addgene_31114 Copy
Species: Homo sapiens
Genetic Insert: beta2-spectrin
Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.
The following "mutations" have been located in this construct. The Bennett lab believes they are due to incorrect sequence data originally entered into NCBI; however, please be aware of these in case you are designing primers or doing mutagenesis:
Derivations from published sequence:
2.59 c-->t (silent)
4.23 c-->g (H-->D)
5.99 g-->c (R-->T)
6.3 g-->a (silent)
Please refer to the Addgene NGS result for the fully validated sequence.
Proper citation: RRID:Addgene_31070 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Proper citation: RRID:Addgene_31100 Copy
Species: Homo sapiens
Genetic Insert: HNF1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31101 Copy
Species: Homo sapiens
Genetic Insert: HNF4A P2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590126
Proper citation: RRID:Addgene_31062 Copy
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