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Species: Other
Genetic Insert: NGFR-P2A-nLAP(EGFP)
Vector Backbone Description: Vector Backbone:pBluescript KS-; Vector Types:Bacterial Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36444289
Proper citation: RRID:Addgene_194506 Copy
Species: Other
Genetic Insert: cLAP(EGFP)-P2A-NGFR
Vector Backbone Description: Vector Backbone:pBluescript KS-; Vector Types:Bacterial Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36444289
Proper citation: RRID:Addgene_194508 Copy
Species: Other
Genetic Insert: cLAP(EGFP)-P2A-NGFR
Vector Backbone Description: Vector Backbone:pBluescript KS-; Vector Types:Bacterial Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36444289
Proper citation: RRID:Addgene_194509 Copy
Species: Other
Genetic Insert: cLAP(EGFP)-P2A-NGFR
Vector Backbone Description: Vector Backbone:pBluescript KS-; Vector Types:Bacterial Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36444289
Proper citation: RRID:Addgene_194511 Copy
Species: Other
Genetic Insert: CUL3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/4AP2/
Proper citation: RRID:Addgene_53673 Copy
Species: Other
Genetic Insert: AK5A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pLIC-SGC1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: T7-lacl regulated, N-terminal his tag, TEV cleavage, LIC cloning using BseRI/ T4 pol, pET backbone. http://www.thesgc.org/structures/2BWJ/
Proper citation: RRID:Addgene_53674 Copy
Species: Other
Genetic Insert: FKBP1AA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/3MDY/
Proper citation: RRID:Addgene_53671 Copy
Species: Other
Genetic Insert: ASB9A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/2XAI/
Proper citation: RRID:Addgene_53670 Copy
Species: Other
Genetic Insert: ZFYVE9C
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BKW/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53618 Copy
Species: Other
Genetic Insert: MTRA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4CCZ/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53628 Copy
Species: Other
Genetic Insert: GAKA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-H102; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: N-terminal His10-TEV;Mhhhhhhhhhhdlgtenlyfqsm http://www.thesgc.org/structures/4C59/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53629 Copy
Species: Other
Genetic Insert: BRD2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4MR5/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53626 Copy
Species: Other
Genetic Insert: PCBD2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4C45/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53624 Copy
Species: Other
Genetic Insert: ETV4A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4CO8/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53638 Copy
Species: Other
Genetic Insert: SP100A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pSUMO-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-SUMO tags, SUMO protease, LIC cloning using BsaI digest/T4 polymerase but non-standard LIC sites, SacB stuffer fragment. Optional non-cleavable biotinylation and His6 tags if reverse primer does not encode stop codon. NB. SUMO recognition sequence is a dummy entry. http://www.thesgc.org/structures/4PTB/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53635 Copy
Species: Other
Genetic Insert: SHKBP1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4CRH/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53636 Copy
Species: Other
Genetic Insert: PRKCBP1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-ZB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Z-Basic solubility-enhancing tag, normal LIC cloning, TEV cleavage. Pavel EXP-09-AC5269 http://www.thesgc.org/structures/4COS/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53633 Copy
Species: Other
Genetic Insert: CBSA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4COO/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53634 Copy
Species: Other
Genetic Insert: CRK7A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV cleavable. http://www.thesgc.org/structures/4CJY/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53643 Copy
Species: Other
Genetic Insert: GBE1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/4BZY/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53640 Copy
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