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Species: Other
Genetic Insert: dnaQ926 dam seqA ugi AID
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69675 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA yfjY
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69674 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA ugi AID (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69684 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdEF
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69683 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdA(A301V)B
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69681 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA emrR ugi APOBEC1 (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69688 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA emrR ugi AID (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69687 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA ugi CDA1 (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69686 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:Hy_pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69911 Copy
Species: Other
Genetic Insert: Tb427tmp.01.1830:MAC054-B05:C234722
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/5C8G for more information
Proper citation: RRID:Addgene_69968 Copy
Species: Other
Genetic Insert: PF13_0238:MAC052-B07:C233961
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/4YY8 for more information
Proper citation: RRID:Addgene_69961 Copy
Species: Other
Genetic Insert: Transposon Tn5-692
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:12218043
Comments: Tn5 derivative Tn5-692 (in plasmid pRL692; GenBank accession no. AF424805) contains a pMB1 oriV; and bears mutations (Zhou, et al. 1998 J. Mol. Biol. 276:913–925) that increase its rate of transposition ca. 100-fold relative to that of Tn5-1058 (Wolk, et al. 1991. Proc. Natl. Acad. Sci. USA 88:5355–5359), providing large numbers of transposon mutants. The erythromycin-resistance gene came from Horinouchi and Weisblum (J. Bacteriol. 150: 804-814, 1982) and the streptomycin-spectinomycin gene came from the Ω-fragment described by Prentki and Krisch (Gene 29, 303-313, 1984) and was purchased as an EcoRI fragment from Amersham.
NOTE--Because Tn5-692 appears able to transpose repeatedly, the user is cautioned not to delay storing strains whose phenotype is of interest.
Additional references:
Miyagishima S-y, Wolk CP, Osteryoung KW (2005) Identification of cyanobacterial cell division genes by comparative and mutational analyses. Mol Microbiol 56: 126-143
Proper citation: RRID:Addgene_70284 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71224 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71225 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71227 Copy
Species: Other
Genetic Insert: dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
Vector Backbone Description: Backbone Size:9200; Vector Backbone:3rd Generation lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29635374
Proper citation: RRID:Addgene_71219 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71231 Copy
Species: Other
Genetic Insert: Tq2CFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26644504
Comments: Please, note that there is no stop codon after the
ER retention signal HDEL, and therefore, it is advisable to use a
terminator in the 3rd box containing stop adjacent to the attR2 site
(such as p2R3a-nosT or p2R3a-3AT)
Proper citation: RRID:Addgene_71264 Copy
Species: Other
Genetic Insert: VenusYFP
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71269 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR/Zeo; Vector Types:Gateway Cloning; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71266 Copy
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