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Species: Other
Genetic Insert: Beta-glucuronidase
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71267 Copy
Species: Other
Genetic Insert: nopaline synthase terminator
Vector Backbone Description: Backbone Marker:ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy; Vector Backbone:p2RP3-pGEMteasy; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26644504
Proper citation: RRID:Addgene_71272 Copy
Species: Other
Genetic Insert: 2xPCP-2xGFP
Vector Backbone Description: Vector Backbone:pHsp83; Vector Types:Insect Expression, P element-mediated germline transformation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_71243 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Proper citation: RRID:Addgene_71235 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71233 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Proper citation: RRID:Addgene_71234 Copy
Species: Other
Genetic Insert: IKBKB
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: CGAG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-C2-STOP
Proper citation: RRID:Addgene_64609 Copy
Species: Other
Genetic Insert: n/a
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCGA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X2-V5
Proper citation: RRID:Addgene_64649 Copy
Species: Other
Genetic Insert: n/a
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCGA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X2-STOP
Proper citation: RRID:Addgene_64648 Copy
Species: Other
Genetic Insert: n/a
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TACT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-X1-V5
Proper citation: RRID:Addgene_64647 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65143 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6576.
Additional article references: Le Guiner et al. Mol. Ther. 22:1923-1935 (2014)
Proper citation: RRID:Addgene_65216 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6871.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6569.
Proper citation: RRID:Addgene_65213 Copy
Species: Other
Genetic Insert: VSV-G
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: cytoplasmic tagging
Proper citation: RRID:Addgene_65301 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pSMART HcKan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65316 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pSMART HcKan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65315 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pSMART HcKan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65314 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65326 Copy
Species: Other
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pPC047; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the ccdB gene was used for screening positive transformants(if inserts failed to replace ccdB, cells would not grow in DH5alpha)
Proper citation: RRID:Addgene_65328 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65325 Copy
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