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Species: Homo sapiens
Genetic Insert: DNM2-mRuby2
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5616; Vector Backbone:pRS424; Vector Types:Bacterial Expression, Yeast Expression, yeast-E.coli shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29668686
Comments: Created using DNA assembly in yeast
Proper citation: RRID:Addgene_107793 Copy
Species: Chloromonas oogama
Genetic Insert: soCoChR-mCardinal
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29184208
Comments: GenBank IDs: soCoChR = MF795583, KA2(1-150) = MF795584
Proper citation: RRID:Addgene_107713 Copy
Species: dicot
Genetic Insert: Cas13a
Vector Backbone Description: Vector Backbone:pMOD_A; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28976959
Comments: This is a MOD_A plasmid compatible with the Cermek et al. toolkit.
https://www.addgene.org/browse/article/28189956/
Proper citation: RRID:Addgene_107691 Copy
Species: Rattus norvegicus
Genetic Insert: dLbCpf1-eBE-YE
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Comments: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)
Proper citation: RRID:Addgene_107689 Copy
Species: Rattus norvegicus
Genetic Insert: dLbCpf1-eBE
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Comments: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)
Proper citation: RRID:Addgene_107688 Copy
Species: Rattus norvegicus
Genetic Insert: dLbCpf1-BE-YEE
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Comments: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)
Proper citation: RRID:Addgene_107687 Copy
Species: Rattus norvegicus
Genetic Insert: dLbCpf1-BE-YE
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Comments: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)
Proper citation: RRID:Addgene_107686 Copy
Species: Rattus norvegicus
Genetic Insert: dLbCpf1-BE
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Comments: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)
Proper citation: RRID:Addgene_107685 Copy
Species: Rattus norvegicus
Genetic Insert: dLbCpf1-BE0ΔiNLS
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Comments: derived from plasmid pCMV-BE3 (David Liu, Addgene plasmid # 73021)
Proper citation: RRID:Addgene_107684 Copy
Vector Backbone Description: Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Proper citation: RRID:Addgene_107683 Copy
Vector Backbone Description: Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553573
Proper citation: RRID:Addgene_107682 Copy
Species: Homo sapiens
Genetic Insert: MAPRE3 gRNA #1 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107729 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1 gRNA #1 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107726 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10768 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10775 Copy
Species: Chloromonas oogama
Genetic Insert: soCoChR-GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29184208
Comments: GenBank IDs: soCoChR = MF795583, KA2(1-150) = MF795584
Proper citation: RRID:Addgene_107709 Copy
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid was previously available as AV-9-PV4365 from the Penn Vector Core. GCaMP6s was created as part of the GENIE project at Janelia Research Campus.
Proper citation: RRID:Addgene_107790 Copy
Species: Synthetic
Genetic Insert: QuasAr3-P2A-CheRiff
Vector Backbone Description: Backbone Size:4401; Vector Backbone:pAAV_hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043747
Proper citation: RRID:Addgene_107700 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:3500; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29551490
Proper citation: RRID:Addgene_107707 Copy
Species: Synthetic
Genetic Insert: paQuasAr3
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pAAV-CAG-FLEX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043747
Proper citation: RRID:Addgene_107702 Copy
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