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Species: Homo sapiens
Genetic Insert: K-Ras
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21706014
Comments: Do NOT use this plasmid directly for transfections, you will need to recombine it into a destination vector.
A set of 4 custom lentiviral vectors is available (http://www.addgene.org/25895, http://www.addgene.org/25896, http://www.addgene.org/25897, http://www.addgene.org/25890) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_31200 Copy
Vector Backbone Description: Backbone Size:4553; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:21238944
Proper citation: RRID:Addgene_31288 Copy
Species: Homo sapiens
Genetic Insert: ANLN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pLenti 6/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21741599
Comments: Please note that the ANLN insert in this plasmid is based on GenBank entry BC034692, rather than the full-length transcript (GenBank entry NM_018685.2).
Proper citation: RRID:Addgene_31203 Copy
Species: Mus musculus
Genetic Insert: Cry1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21613214
Proper citation: RRID:Addgene_31282 Copy
Species: Homo sapiens
Genetic Insert: HMGB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pLenti6/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21741599
Proper citation: RRID:Addgene_31208 Copy
Species: E. coli
Genetic Insert: RecG-EGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3992; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23110454
Proper citation: RRID:Addgene_31274 Copy
Species: E. coli
Genetic Insert: RecG-ST2-TaV-EGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3992; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23110454
Proper citation: RRID:Addgene_31275 Copy
Species: Homo sapiens
Genetic Insert: TopBP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5-FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502314
Proper citation: RRID:Addgene_31313 Copy
Genetic Insert: GCGFP
Vector Backbone Description: Backbone Size:2316; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_31266 Copy
Genetic Insert: ECFP(loxP)EYFP
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12682379
Comments: Cre reporter: from blue fluorescence to yellow fluorescence
Proper citation: RRID:Addgene_31306 Copy
Species: Homo sapiens
Genetic Insert: Mesothelin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:33000; Vector Backbone:pAdEasy; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21505261
Comments: Msln Promoter sequence provided by author (see above link)
Please note that Addgene was unable to sequence verify this plasmid. The depositing laboratory PCR amplified two regions of this plasmid using the following primer pairs and sequenced the resulting PCR products to confirm the plasmid.
Msln-1F: TCATTTGTTCCCTTTGACGGC
Msln-1R: CTCTCCCCAGCATCCACATTC
Expect 987 nt product
Msln-2F: TTGCCTACAACACCCTGCTGCG
Msln-2R: GCTCACAATGCTTCCATCAAACG
Expect 792 nt product
Proper citation: RRID:Addgene_31305 Copy
Species: Homo sapiens
Genetic Insert: Human Homologue of Ariadne (HHARI)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21532592
Comments: Capili AD, Edghill EL, Wu, K and Borden KL (2004)Structure of the C-terminal RING Finger from a RING-IBR-RING/TRIAD Motif Reveals a Novel Zinc-binding Domain Distinct from a RING. J. Mol. Bio. 340:1117-29.
Proper citation: RRID:Addgene_31254 Copy
Species: Homo sapiens
Genetic Insert: Huntingtin-interacting protein 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12163454
Proper citation: RRID:Addgene_31253 Copy
Species: Mus musculus
Genetic Insert: Clock
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pcDNA4a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21613214
Proper citation: RRID:Addgene_31366 Copy
Species: Mus musculus
Genetic Insert: Bmal1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21613214
Proper citation: RRID:Addgene_31367 Copy
Species: Homo sapiens
Genetic Insert: Brd4FL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21555454
Proper citation: RRID:Addgene_31351 Copy
Species: Homo sapiens
Genetic Insert: Brd4 444-722
Vector Backbone Description: Backbone Size:5000; Vector Backbone:MSCV-CMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21555454
Proper citation: RRID:Addgene_31353 Copy
Species: Homo sapiens
Genetic Insert: Brd4 1224-1362
Vector Backbone Description: Backbone Size:5000; Vector Backbone:MSCV-CMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21555454
Proper citation: RRID:Addgene_31355 Copy
Species: Homo sapiens
Genetic Insert: Brd4 1047-1362
Vector Backbone Description: Backbone Size:5000; Vector Backbone:MSCV-CMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21555454
Proper citation: RRID:Addgene_31354 Copy
Species: Homo sapiens
Genetic Insert: JMJD6
Vector Backbone Description: Backbone Size:5000; Vector Backbone:MSCV-CMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21555454
Proper citation: RRID:Addgene_31358 Copy
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