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Species: Other
Genetic Insert: DPYSL3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-6HZB; Vector Types:Baculovirus transfer vector; Bacterial Resistance:Ampicillin
Comments: Baculovirus (Bac to bac). N-terminal His6-ZB-TEV tag. Clone using BseRI and standard Lic extensions http://www.thesgc.org/structures/4BKN/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53641 Copy
Species: Other
Genetic Insert: MAT2AA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2P02/
Proper citation: RRID:Addgene_53648 Copy
Species: Other
Genetic Insert: HPDA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/3ISQ/
Proper citation: RRID:Addgene_53653 Copy
Species: Other
Genetic Insert: ELAC1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/3ZWF/
Proper citation: RRID:Addgene_53654 Copy
Species: Other
Genetic Insert: HTD2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/3IR3/
Proper citation: RRID:Addgene_53652 Copy
Species: Other
Genetic Insert: SNX24A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AZ9/
Proper citation: RRID:Addgene_53656 Copy
Species: Other
Genetic Insert: JMJD1CA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/2YPD/
Proper citation: RRID:Addgene_53664 Copy
Species: Other
Genetic Insert: FBXL11A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BBQ/
Proper citation: RRID:Addgene_53660 Copy
Species: Other
Genetic Insert: alpha NTD-Beta flap fusion
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details.
This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53734 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24656821
Proper citation: RRID:Addgene_53965 Copy
Species: Other
Genetic Insert: E1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Hepatitis C Viral Coating Protein (E1). Excitation = 487; Emission = 509. Localization data: Coat Protein
Proper citation: RRID:Addgene_54071 Copy
Species: Other
Genetic Insert: RabL5
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 487; Emission = 509. Localization data: Ras GTPase
Proper citation: RRID:Addgene_54246 Copy
Species: Other
Genetic Insert: SV40 T-Antigen Nuclear Localization Signal (NLS)
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mEmerald-Nucleus; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: SV40 T-Antigen Nuclear Localization Signal (NLS). Excitation = 487; Emission = 509. Localization data: Nucleus
Proper citation: RRID:Addgene_54206 Copy
Species: Other
Genetic Insert: VSVG
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Addgene sequencing results find R26Q, F204S, and E210G in vsv-G compared to reference sequence emb|CAM84176.1. Excitation = 487; Emission = 509. Localization data: Golgi/Endoplasmic Reticulum
Proper citation: RRID:Addgene_54307 Copy
Species: Other
Genetic Insert: mCherry and BlasticidinS-resistance
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBS SK(+); Vector Types:Mammalian Expression, Mouse Targeting, CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28421278
Proper citation: RRID:Addgene_54321 Copy
Species: Other
Genetic Insert: lacZ A-B
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54457 Copy
Species: Other
Genetic Insert: nopaline synthase promoter
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54456 Copy
Species: Other
Genetic Insert: ccdB C-D
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24551083
Proper citation: RRID:Addgene_54460 Copy
Species: Other
Genetic Insert: CytERM
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cytochrome p450 . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54882 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:5500; Vector Backbone:mCherry-miniSOG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21483721
Comments: . Excitation = 448 / 473; Emission = 500 / 528
Proper citation: RRID:Addgene_55094 Copy
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