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Species: Homo sapiens
Genetic Insert: PP2A regulatory subunit B, beta isoform
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGAGGAGGACATTGATACC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GTTAACCTTGTCCTGGAATATA-3'.
Proper citation: RRID:Addgene_16181 Copy
Species: Homo sapiens
Genetic Insert: PRKC gamma
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16713569
Comments: Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGCTGGTCTGGGCCCC-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG CATGACGGGCACAGGCACT-3'.
Proper citation: RRID:Addgene_16180 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Prpl-3 | histamine | rpl-3 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33437931
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159796 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159794 Copy
Vector Backbone Description: Backbone Size:9875; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD118.60, Ligation number L3808.
Proper citation: RRID:Addgene_1598 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159801 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15974 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159813 Copy
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pFA6a-5FLAG-KanMX6; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18729046
Comments: Template vector for a PCR based gene-tagging (5 x FLAG tag) method in S. pombe.
Proper citation: RRID:Addgene_15983 Copy
Species: Mus musculus
Genetic Insert: Th
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159901 Copy
Species: Homo sapiens
Genetic Insert: GSK3beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10364240
Proper citation: RRID:Addgene_15994 Copy
Species: Rattus norvegicus
Genetic Insert: AMPK alpha2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11389854
Proper citation: RRID:Addgene_15992 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590437
Proper citation: RRID:Addgene_15990 Copy
Species: Mus musculus
Genetic Insert: Penk
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159916 Copy
Species: Mus musculus
Genetic Insert: Rosa26 gRNA
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159914 Copy
Species: Synthetic
Genetic Insert: NFPnls
Vector Backbone Description: Vector Backbone:pPB-MCSfwd; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33453576
Comments: The Brainbow3.2 cassette was derived from pThy1-Brainbow3.2 (https://www.addgene.org/45179/).
Addgene QC identified several ambiguous bases in the backbone sequence that could not be resolved by standard NGS. These ambiguities are present in the backbone sequence and are not of concern for the function of the construct.
Proper citation: RRID:Addgene_159928 Copy
Species: Homo sapiens
Genetic Insert: RPL10a
Vector Backbone Description: Backbone Size:6909; Vector Backbone:pSicoR-eIF1a-mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209477
Proper citation: RRID:Addgene_159890 Copy
Species: Homo sapiens
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590437
Comments: The PH domain of AKT1 has been deleted.
Proper citation: RRID:Addgene_15989 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18583990
Comments: Full length Rb coding sequence, amino acids 1-928. The Rb sequence comprised between sites BamHI and SalI was cloned between BglII and SalI sites in pEGFP-C1.
Proper citation: RRID:Addgene_16004 Copy
Species: Homo sapiens
Genetic Insert: Akt2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1(+)/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: There is a silent mutation at Arg552 (CGA to CGG).
Proper citation: RRID:Addgene_16000 Copy
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