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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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DPYSL3A-c010 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53641 | DPYSL3A | Other | Ampicillin | Baculovirus (Bac to bac). N-terminal His6-ZB-TEV tag. Clone using BseRI and standard Lic extensions http://www.thesgc.org/structures/4BKN/ Use BL21(DE3)-R3-pRARE2 strain for expression. | Backbone Marker:SGC; Vector Backbone:pFB-6HZB; Vector Types:Baculovirus transfer vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:32 | 1 | ||
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MAT2AA-c001 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53648 | MAT2AA | Other | Kanamycin | T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2P02/ | Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-15 01:16:32 | 1 | |
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HPDA-c103 Resource Report Resource Website |
RRID:Addgene_53653 | HPDA | Other | Kanamycin | C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/3ISQ/ | Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-15 01:16:32 | 0 | |
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ELAC1A-c005 Resource Report Resource Website |
RRID:Addgene_53654 | ELAC1A | Other | Kanamycin | C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/3ZWF/ | Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-15 01:16:34 | 0 | |
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HTD2A-c022 Resource Report Resource Website |
RRID:Addgene_53652 | HTD2A | Other | Kanamycin | T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/3IR3/ | Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-15 01:16:32 | 0 | |
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SNX24A-c003 Resource Report Resource Website |
RRID:Addgene_53656 | SNX24A | Other | Kanamycin | T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AZ9/ | Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-15 01:16:32 | 0 | |
|
JMJD1CA-c257 Resource Report Resource Website |
RRID:Addgene_53664 | JMJD1CA | Other | Kanamycin | C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/2YPD/ | Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments. K2381N | 2026-08-15 01:16:32 | 0 | |
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FBXL11A-c316 Resource Report Resource Website |
RRID:Addgene_53660 | FBXL11A | Other | Kanamycin | T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BBQ/ | Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-15 01:16:34 | 0 | |
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pBRα-β flap (831-1057) Resource Report Resource Website 1+ mentions |
RRID:Addgene_53734 | alpha NTD-Beta flap fusion | Other | Ampicillin | PMID:9121589 | Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details. This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 1 | |
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pcDNA5-FRT-GFP-mCherry-3pGW Resource Report Resource Website |
RRID:Addgene_53965 | GFP | Other | Chloramphenicol and Ampicillin | PMID:24656821 | Backbone Marker:Life Technologies; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:16:35 | 0 | ||
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mEmerald-E1-C-18 Resource Report Resource Website |
RRID:Addgene_54071 | E1 | Other | Kanamycin | Hepatitis C Viral Coating Protein (E1). Excitation = 487; Emission = 509. Localization data: Coat Protein | Backbone Size:4750; Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:36 | 0 | ||
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mEmerald-RabL5-N Resource Report Resource Website |
RRID:Addgene_54246 | RabL5 | Other | Kanamycin | . Excitation = 487; Emission = 509. Localization data: Ras GTPase | Backbone Size:4750; Vector Backbone:mEmerald-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:38 | 0 | ||
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mEmerald-Nucleus-7 Resource Report Resource Website 1+ mentions |
RRID:Addgene_54206 | SV40 T-Antigen Nuclear Localization Signal (NLS) | Other | Kanamycin | SV40 T-Antigen Nuclear Localization Signal (NLS). Excitation = 487; Emission = 509. Localization data: Nucleus | Backbone Size:4800; Vector Backbone:mEmerald-Nucleus; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:38 | 4 | ||
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mEmerald-VSVG-16 Resource Report Resource Website 1+ mentions |
RRID:Addgene_54307 | VSVG | Other | Kanamycin | Addgene sequencing results find R26Q, F204S, and E210G in vsv-G compared to reference sequence emb|CAM84176.1. Excitation = 487; Emission = 509. Localization data: Golgi/Endoplasmic Reticulum | Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:39 | 1 | ||
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pBS SK mCherryROSAbsr Resource Report Resource Website |
RRID:Addgene_54321 | mCherry and BlasticidinS-resistance | Other | Ampicillin | PMID:28421278 | Backbone Size:2961; Vector Backbone:pBS SK(+); Vector Types:Mammalian Expression, Mouse Targeting, CRISPR, TALEN; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:39 | 0 | ||
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LI dy Esp3I-lacZ A-B Resource Report Resource Website |
RRID:Addgene_54457 | lacZ A-B | Other | Gentamicin | PMID:24551083 | Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin | 2026-08-15 01:16:40 | 0 | ||
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LI A-B pNOS c1 Resource Report Resource Website |
RRID:Addgene_54456 | nopaline synthase promoter | Other | Gentamicin | PMID:24551083 | Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin | 2026-08-15 01:16:40 | 0 | ||
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LI dy Esp3I-ccdB C-D Resource Report Resource Website |
RRID:Addgene_54460 | ccdB C-D | Other | Gentamicin | PMID:24551083 | Backbone Marker:Parniske lab; Vector Backbone:pUC57 (Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin | 2026-08-15 01:16:40 | 0 | ||
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mApple-CytERM-N-17 Resource Report Resource Website |
RRID:Addgene_54882 | CytERM | Other | Kanamycin | Cytochrome p450 . Excitation = 568; Emission = 592 | Backbone Size:4750; Vector Backbone:mApple-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:44 | 0 | ||
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mCherry-miniSOG-Rab5a-7 Resource Report Resource Website |
RRID:Addgene_55094 | Rab5a | Other | Kanamycin | PMID:21483721 | . Excitation = 448 / 473; Emission = 500 / 528 | Backbone Size:5500; Vector Backbone:mCherry-miniSOG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:46 | 0 |
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