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Species: Synthetic
Genetic Insert: URA-GAL1-fCLIP-GS
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188939 Copy
Species: Synthetic
Genetic Insert: GFPmut3
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Amp + Kan + Dap
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187381 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_187387 Copy
Species: Synthetic
Genetic Insert: IRES-creERT2-FNF
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This IRES-creERT2-FNF cassette was used in the Id1-IRES-creERT2 allele (http://www.informatics.jax.org/allele/MGI:4366863).
Compared to Bochkov and Palmenberg, 2006 (https://doi.org/10.2144/000112243), this EMCV IRES has a 1-bp deletion and an additional base of A ("7A"). The reason for these changes is unclear. These changes were in the electronic sequence file I received. The actual DNA sequence of the IRES was the same as the electronic sequence file. I incorporated this IRES into my plasmids without introducing additional changes. It was more recently tested in driving tdTomato expression (see https://www.addgene.org/185966/), and it was functional. I did not change those bases back and compare whether the expression level is then higher or lower. It may or may not be attenuated compared to the Bochkov and Palmenberg EMCV IRES.
Proper citation: RRID:Addgene_188230 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pkD4; Vector Types:Bacterial Expression; Bacterial Resistance:Amp + Kan + Dap
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187386 Copy
Species: Synthetic
Genetic Insert: 3XHA-fCLIP-Hygromycin
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188919 Copy
Species: Synthetic
Genetic Insert: 3XHA-fSNAP-NAT
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Proper citation: RRID:Addgene_188916 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Víctor de Lorenzo; Backbone Size:3730; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36511859
Proper citation: RRID:Addgene_188964 Copy
Species: Synthetic
Genetic Insert: ade2 KO CRISPEY cassette
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37082145
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.05.494888v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_187912 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167903 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4124; Vector Backbone:pTrc99A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36511859
Proper citation: RRID:Addgene_188967 Copy
Species: Synthetic
Genetic Insert: Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
Vector Backbone Description: Backbone Marker:GeneArt (Thermo Fisher Scientific); Backbone Size:2414; Vector Backbone:pOA-RQ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36220271
Proper citation: RRID:Addgene_186030 Copy
Species: Synthetic
Genetic Insert: mApple
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_187690 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167870 Copy
Species: Synthetic
Genetic Insert: loxP-eGFP-attP-Bsd-lox251
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183751 Copy
Species: Synthetic
Genetic Insert: loxP-eGFP-attP-Bsd-lox257
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183754 Copy
Species: Synthetic
Genetic Insert: FRT-mCherry-attP-BleoR-F3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183755 Copy
Species: Synthetic
Genetic Insert: FRT-mCherry-attP-BleoR-F3
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36313798
Proper citation: RRID:Addgene_183752 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167894 Copy
Species: Synthetic
Genetic Insert: 3XHA-HALO-NAT
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35944214
Comments: This constructed is intended for use as a template for PCR and not for expression of the insert.
Proper citation: RRID:Addgene_188931 Copy
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