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Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167919 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167917 Copy
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167916 Copy
Species: Synthetic
Genetic Insert: EKAREN4
Vector Backbone Description: Backbone Size:3450; Vector Backbone:miniTol2 expression vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. The generation of stable cell lines using this plasmid requires the co-transfection of a transposase-expressing plasmid. The depositing lab recommends the use of Plasmid 158774.
Proper citation: RRID:Addgene_167824 Copy
Species: Synthetic
Genetic Insert: EKAREN4
Vector Backbone Description: Vector Backbone:pLV lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis.
Proper citation: RRID:Addgene_167829 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167896 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167895 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167932 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene Plasmid #52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33622708
Proper citation: RRID:Addgene_167930 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167892 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:pB-CAGGS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167890 Copy
Species: Synthetic
Genetic Insert: dxCas9(3.7)-VPR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: dxCas9(3.7)-VPR is cloned from dxCas(3.7)-VPR (Addgene Plasmid #108383).
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167937 Copy
Species: Synthetic
Genetic Insert: KRAB-dCas9-DHFR
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: KRAB-dCas9 cloned from pAAVS1-NDi-CRISPRi (Gen1) (Addgene Plasmid #73497).
Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167935 Copy
Species: Homo sapiens
Genetic Insert: CORO6
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: SGC link: http://www.thesgc.org/structures/7KYX/
Proper citation: RRID:Addgene_167845 Copy
Species: Homo sapiens
Genetic Insert: WDR55
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: SGC link: http://www.thesgc.org/structures/7KQQ/
Proper citation: RRID:Addgene_167844 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Transcription: linearize with Not I and transcribe with SP6.
Plasmid History:
Constructed by Randall T. Moon.
Notes for Use:
Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
Addgene QC found 2 point mutations, T511A and T717S, that do not affect function.
Proper citation: RRID:Addgene_16788 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-PDZ
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10427095
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996)
was engineered to now contain Dsh amino acids 1-267 and 381-736 (deleting 268-380).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16786 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-COOH
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10427095
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-588 (deleting 599-736).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16784 Copy
Species: Synthetic
Genetic Insert: EKAREN4(TA)
Vector Backbone Description: Vector Backbone:pLV lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33795873
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis.
Proper citation: RRID:Addgene_167830 Copy
Species: Homo sapiens
Genetic Insert: TETv4 (TET1CD-XTEN80-dCas9)
Vector Backbone Description: Backbone Size:5574; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33838111
Proper citation: RRID:Addgene_167983 Copy
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