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Species: Synthetic
Genetic Insert: Stabilized hairpin of miR-let7d
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: let7d_stbC motif was inserted between BamHI and AgeI sites of pAptamerCassette-EGFP (ID: 140288).
Proper citation: RRID:Addgene_140266 Copy
Species: Homo sapiens
Genetic Insert: LIN28A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: CDS of LIN28A was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140267 Copy
Species: Synthetic
Genetic Insert: Two PP7CP-binding sites embedded in scaffold
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: scPP7(2xWT) motif was inserted between BamHI and AgeI sites of pAptamerCassette-EGFP (ID: 140288).
Proper citation: RRID:Addgene_140261 Copy
Species: Homo sapiens
Genetic Insert: U1A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of WT U1A was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140264 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage MS2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of mutant MS2CP (MS2CP(V29I)) was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140260 Copy
Species: Homo sapiens
Genetic Insert: Truncated U1A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Comments: CDS of Truncated U1A was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140269 Copy
Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Proper citation: RRID:Addgene_140278 Copy
Species: Synthetic
Genetic Insert: BoxCD
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20016495
Comments: Alternative name: p1-boxC/D-EGFP
Proper citation: RRID:Addgene_140272 Copy
Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Proper citation: RRID:Addgene_140288 Copy
Species: Rattus norvegicus
Genetic Insert: CREB1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31883788
Proper citation: RRID:Addgene_137003 Copy
Species: Rattus norvegicus
Genetic Insert: KIX domain
Vector Backbone Description: Backbone Size:4000; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31883788
Proper citation: RRID:Addgene_137005 Copy
Species: Synthetic
Genetic Insert: (GGS)2G
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137097 Copy
Species: Synthetic
Genetic Insert: (GGS)4GSG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137099 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans GLO-optimized GFP
Vector Backbone Description: Backbone Size:2540; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR, SapTrap donor for cloning repair templates for C. elegans; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137021 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans GLO-optimized Halo Tag
Vector Backbone Description: Backbone Size:2540; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR, SapTrap donor for cloning repair templates for C. elegans; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137023 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66635.2
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.
Proper citation: RRID:Addgene_137046 Copy
Genetic Insert: blasticidin-S deaminase
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Leishmania expression gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137049 Copy
Species: Other
Genetic Insert: puromycin N-acetyl-transferase
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Leishmania expression gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137050 Copy
Vector Backbone Description: Vector Backbone:GOI flox pDONR221; Vector Types:Leishmania floxed gene expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137055 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAF07416.1
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli. RevA is a lipoprotein. RevA is not expected to be lipidated in this construct. Following TEV cleavage, RevA lacks 5 residues (CKAYV) at the N-terminus of the mature protein.
Proper citation: RRID:Addgene_137064 Copy
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