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Species: Homo sapiens
Genetic Insert: protein phosphatase 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31331 Copy
Species: Homo sapiens
Genetic Insert: PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31332 Copy
Species: Homo sapiens
Genetic Insert: PRDX2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31338 Copy
Species: Homo sapiens
Genetic Insert: HNF4A 3'UTR (long)
Vector Backbone Description: Backbone Size:6380; Vector Backbone:pRL-Con; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22140441
Comments: To measure regulatory potential of 3'UTR
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31445 Copy
Species: Homo sapiens
Genetic Insert: stathmin 1/oncoprotein 18
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Please note that Addgene's quality control sequence shows a small deletion in the vector region downstream of insert; the deletion does not affect any features in this construct.
Proper citation: RRID:Addgene_31326 Copy
Species: Homo sapiens
Genetic Insert: TopBP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502314
Proper citation: RRID:Addgene_31317 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3501; Vector Backbone:phrGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19105832
Proper citation: RRID:Addgene_31385 Copy
Genetic Insert: rtTA-IRES-BirA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:33000; Vector Backbone:pAdEasy; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20890903
Proper citation: RRID:Addgene_31375 Copy
Genetic Insert: ECFP(loxP)MCS
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Cre/Lox, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11376165
Comments: Cre and Flp reporter: from blue protein fluorescence to gene of interested inserted into multiple cloning site (MCS)
Proper citation: RRID:Addgene_31377 Copy
Genetic Insert: Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31483 Copy
Genetic Insert: Traffic Light Reporter 1.1 (Sce Target) EF Puro
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31482 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans glutamate-gated chloride channel alpha, crystallized construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pFastbac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21572436
Proper citation: RRID:Addgene_31488 Copy
Genetic Insert: Delta-selective C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20959447
Proper citation: RRID:Addgene_31526 Copy
Species: Homo sapiens
Genetic Insert: YFP-HA-Protein Kinase D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19581308
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It should work fine with the 12CA5 HA antibody.
Proper citation: RRID:Addgene_31527 Copy
Species: Synthetic
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2760; Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21631110
Proper citation: RRID:Addgene_31472 Copy
Species: Homo sapiens
Genetic Insert: preimplantation protein 3
Vector Backbone Description: Backbone Size:3460; Vector Backbone:pQStrep2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: Please note that there is a small gap between Addgene's quality control sequence and the reference sequence provided by the depositor. The gap is downstream of the ORF and should not affect function.
Proper citation: RRID:Addgene_31592 Copy
Species: Synthetic
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:British Biotechnology; Backbone Size:5300; Vector Backbone:GW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21631110
Proper citation: RRID:Addgene_31473 Copy
Genetic Insert: SFFV d14 GFP donor EF1s I-SceI
Vector Backbone Description: Backbone Size:5092; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31476 Copy
Genetic Insert: SFFV d14GFP
Vector Backbone Description: Backbone Size:5516; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31475 Copy
Species: fluorescent proteins
Genetic Insert: eYFP and mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:2850; Vector Backbone:pTRETightBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21857679
Comments: 4 tandem miR-20 binding sites in mCherry 3'UTR
Proper citation: RRID:Addgene_31465 Copy
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