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Species: Mus musculus
Genetic Insert: mFZ6-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: The clone mFZ4/pRK5 from Nathans lab (accession # U43319), was used as template to PCR amplify just the coding region as an EcoR1 insert. The 3' stop was kept.
Constructed by: Rebecca Bates/Randall Moon.
Notes for Use:
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16802 Copy
Species: Homo sapiens
Genetic Insert: CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
Vector Backbone Description: Backbone Size:4836; Vector Backbone:CAG expression plasmid; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33838111
Proper citation: RRID:Addgene_167981 Copy
Species: Mus musculus
Genetic Insert: FZ7
Vector Backbone Description: Backbone Size:4095; Vector Backbone:PCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: 3 Kb 5' EcoRI - 3' XbaI insert of mFZ7/pRK5 (Nathans), with engineered optimal kozak sequence.
Plasmid History:
Species of Sequence Origin: Xenopus.
Constructed by: Rebecca Bates/Randall Moon.
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16801 Copy
Species: Mus musculus
Genetic Insert: Hrasls3
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-IPU-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_167987 Copy
Species: Homo sapiens
Genetic Insert: SRY-Box Transcription Factor 9
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167972 Copy
Species: Homo sapiens
Genetic Insert: Tp63
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167971 Copy
Species: Synthetic
Genetic Insert: Ykt6(L161-L177)-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_167997 Copy
Species: Synthetic
Genetic Insert: VAMP1(T29-K89)-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_167996 Copy
Species: Synthetic
Genetic Insert: eLACCO1.1
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34873165
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167944 Copy
Species: Homo sapiens
Genetic Insert: SFTPC
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167943 Copy
Species: Homo sapiens
Genetic Insert: TTF1
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167942 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_167966 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_167964 Copy
Species: Synthetic
Genetic Insert: Dre recombinase
Vector Backbone Description: Vector Backbone:UC57; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34612202
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_167968 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y705E, Y706E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_167955 Copy
Species: Synthetic
Genetic Insert: Ykt6(L161-L177)-T3'PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168003 Copy
Species: Synthetic
Genetic Insert: PTEN-T7PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168005 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz1-HA-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.
Comments: Rfz1 from Henk Roelink. This sequence has a few differences.
from the published sequence. Sites listed in Rfz1 are not a complete list.
and I haven't searched through the CS2+ sequence to see if/where those sites.
are present. HA tag version made by Jeff Brown (2/98-3/98) insert the epitope.
into the StyI site (1904). The 3' Sty site is destroyed by the tags, but.
the 5' site is inteact. Epitope can be detected in PBS/Formaldehyde fixed.
tissue permeablized with 0.2% Triton. Frizzled tends to ppt out of SDS sample.
buffer when boiled, so either warm samples to ~70 degrees Celsius for a.
few minutes before loading samples or use a strong denaturing loading buffer.
and incubate the samples at room temp for half and hour before loading.
The StyI site is in the 3rd extracellular loop of the protein.
Proper citation: RRID:Addgene_16797 Copy
Species: Synthetic
Genetic Insert: PTEN-T3'PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168006 Copy
Species: Rattus norvegicus
Genetic Insert: beta 2 AR Fz2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588714
Comments: Chimera of beta 2 adrenergic receptor (extracellular and.
transmembrane domains) and rat frizzled-2 (intracellular). Activated by isoproterenol and inhibited by propranolol.
Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al.,
2000, JBC 275:12701-12711.
Proper citation: RRID:Addgene_16794 Copy
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