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Species: Other
Genetic Insert: GFP(-6)-H2_mCherry(-6)-L2
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205051 Copy
Species: Other
Genetic Insert: MTS-miniTurbo-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214456 Copy
Species: Other
Genetic Insert: MTS-TurboID-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214457 Copy
Species: Other
Genetic Insert: PM-TurboID-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214455 Copy
Species: Other
Genetic Insert: tRNAGln promoter, sU6 promoter, gRNA scaffolds
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_213016 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site C3
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212700 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site E4
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212698 Copy
Species: Other
Genetic Insert: mtkeima
Vector Backbone Description: Vector Backbone:pB-CAG-BGH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34048705
Proper citation: RRID:Addgene_210338 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site F3
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212703 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site C3
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212701 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site F3
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212702 Copy
Species: other
Genetic Insert: dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
Vector Backbone Description: Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38052959
Proper citation: RRID:Addgene_214348 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site E2
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212695 Copy
Species: Other
Genetic Insert: Plastid transit peptide fused mCherry-UnaG
Vector Backbone Description: Vector Backbone:pGWB508; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213862 Copy
Species: Other
Genetic Insert: Peroxisome transit peptide fused mCerulean
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213861 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site E2
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212694 Copy
Species: Other
Genetic Insert: YPet
Vector Backbone Description: Vector Backbone:pHAGE-EFS-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38554702
Proper citation: RRID:Addgene_215747 Copy
Species: Other
Genetic Insert: gRNA targeting to the intergradtion site E4
Vector Backbone Description: Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39260817
Proper citation: RRID:Addgene_212699 Copy
Species: Other
Genetic Insert: sgRNA-RL554 with TTTA stretch
Vector Backbone Description: Vector Backbone:pSuper; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28177825
Proper citation: RRID:Addgene_215863 Copy
Species: other
Genetic Insert: BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
Vector Backbone Description: Vector Backbone:pSC101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38086386
Comments: Please note: Plasmid contains N242S, R257H, and H289Y mutations in Rep101. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_214741 Copy
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