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Species: Synthetic
Genetic Insert: G(TP)4TG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137101 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66700.1
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli. BB0323 is a lipoprotein. BB0323 is not expected to be lipidated in this construct. After TEV cleavage, the target protein has an added A residue at the N-terminus of the mature protein.
Proper citation: RRID:Addgene_137067 Copy
Species: Synthetic
Genetic Insert: GGASPAGG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137102 Copy
Species: Synthetic
Genetic Insert: GGA(EAAAK)2AGG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137104 Copy
Species: Synthetic
Genetic Insert: FKBP12
Vector Backbone Description: Vector Backbone:pFD; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137108 Copy
Species: Synthetic
Genetic Insert: AI(TVMV)
Vector Backbone Description: Vector Backbone:pFD; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137111 Copy
Species: Synthetic
Genetic Insert: GG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137091 Copy
Species: Synthetic
Genetic Insert: GPG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137093 Copy
Species: Synthetic
Genetic Insert: GGSG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137095 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5332; Vector Backbone:pET28a-derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Proper citation: RRID:Addgene_13729 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5312; Vector Backbone:pET28a derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Comments: Construct is expressed with a precission protease cleavable 6xHis tag
Proper citation: RRID:Addgene_13727 Copy
Species: Caenorhabditis elegans
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5317; Vector Backbone:pET28a-derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Proper citation: RRID:Addgene_13728 Copy
Species: Homo sapiens
Genetic Insert: CALCOCO1 R12H
Vector Backbone Description: Vector Backbone:pCMV6-Entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137770 Copy
Species: Rattus norvegicus
Genetic Insert: GRASP65
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137709 Copy
Species: Salmonella enterica serovar Typhimurium
Genetic Insert: SopF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5847; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137734 Copy
Species: Homo sapiens
Genetic Insert: CALCOCO1 W47A
Vector Backbone Description: Vector Backbone:pCMV6-Entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137769 Copy
Species: Bos taurus
Genetic Insert: Bovine GRK2
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137778 Copy
Species: Homo sapiens
Genetic Insert: ITPKA
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137811 Copy
Species: Bos taurus
Genetic Insert: Bovine GRK2[D110A K220R]
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137779 Copy
Species: Homo sapiens
Genetic Insert: Human GRK6[L66A E514A]
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137781 Copy
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