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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pSLIK sh human Rb 1534 hyg
 
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1+ mentions
RRID:Addgene_31500 RB shRNA Homo sapiens Ampicillin The whole sequence for the shRNA including the loop should be from 3872-3931 in the vector: AGCGAAACGATTATCCATTCAAATAGTGAAGCCACAGATGTATTTGAAT The shRNA is downstream of the EGFP GGATAATCGTT pSLIK vector from Ian Frasier Backbone Size:13700; Vector Backbone:pSLIK-EGFP; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin The target sequence for shRB 1534 is GAACGATTATCCATTCAAA 2026-08-15 01:13:35 3
pTRETightBI-RY-7
 
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1+ mentions
RRID:Addgene_31466 eYFP and mCherry fluorescent proteins Ampicillin PMID:21857679 7 tandem miR-20 binding sites in mCherry 3'UTR Backbone Marker:Clontech; Backbone Size:2850; Vector Backbone:pTRETightBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:34 1
pQStrep2-ARPC3
 
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RRID:Addgene_31580 Actin related protein 2/3 complex, subunit 3 Homo sapiens Ampicillin PMID:15998469 The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. Please note that Addgene's quality control sequence shows a small deletion in the vector region downstream of insert; the deletion does not affect any features in this construct. Backbone Size:3460; Vector Backbone:pQStrep2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:33 1
pLS1
 
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RRID:Addgene_31490 lactose repressor protein Ampicillin PMID:11266612 Parent plasmid was pEMBL9' with insert of extended LacI sequence from pIQ (Hare & Sadler, Gene 3 (1978) 269-278. For expression: E. coli BLIM cells (or any E. coli WITHOUT lactose repressor in the genome or accompanying plasmid) Backbone Size:4540; Vector Backbone:pEMBL9'; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:35 2
pTARA
 
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1+ mentions
RRID:Addgene_31491 RNA polymerase T7 phage Chloramphenicol PMID:10610690 For expression: E. coli CV2 N823D mutation should not affect plasmid function. Backbone Size:5400; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol N823D 2026-08-15 01:13:33 5
pOVA
 
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RRID:Addgene_31598 OVA Gallus gallus Ampicillin PMID:9364701 *The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details. Backbone Marker:Reff et al., 1994; Backbone Size:9209; Vector Backbone:TCAE (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:33 2
Snail_pGL2
 
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10+ mentions
RRID:Addgene_31694 Snail Homo sapiens Ampicillin PMID:12705869 Contains Snail promoter (-1045 to -56) sequence upstream of luciferase Backbone Size:5598; Vector Backbone:pGL2_Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:13:38 17
pDEST40-MCU-V5-HIS
 
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1+ mentions
RRID:Addgene_31731 MCU Homo sapiens Ampicillin PMID:21685886 Backbone Marker:Invitrogen; Backbone Size:5485; Vector Backbone:pDEST40-pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:35 4
SnailHA_pcDNA3
 
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1+ mentions
RRID:Addgene_31697 Snail Homo sapiens Ampicillin PMID:15314165 Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:38 5
GFP-Rab5
 
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1+ mentions
RRID:Addgene_31733 Rab5 Drosophila melanogaster Ampicillin PMID:21169635 Backbone Marker:Drosophila Gateway Collection; Vector Backbone:pAGW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:36 9
pcDNA3-ATR WT
 
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10+ mentions
RRID:Addgene_31611 ATR Homo sapiens Ampicillin PMID:16354678 Plasmid contains an R1631H polymorphism. This is not thought to affect plasmid function. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:35 12
pDEST47-MCU-GFP
 
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1+ mentions
RRID:Addgene_31732 MCU Homo sapiens Ampicillin PMID:21685886 *A2T aa residue substitution in MCU, which is not known to affect protein function Backbone Marker:Invitrogen (Cat#V355-20); Backbone Size:5784; Vector Backbone:pcDNA6.2/C-EmGFP-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin * 2026-08-15 01:13:39 7
GFP-Rab8
 
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1+ mentions
RRID:Addgene_31735 Rab8 Drosophila melanogaster Ampicillin PMID:21169635 Backbone Marker:Drosophila Gateway Collection; Vector Backbone:pAGW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 3
GFP-Rab6
 
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1+ mentions
RRID:Addgene_31734 Rab6 Drosophila melanogaster Ampicillin PMID:21169635 Backbone Marker:Drosophila Gateway Collection; Vector Backbone:pAGW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:35 1
GFP-Rab10
 
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1+ mentions
RRID:Addgene_31737 Rab10 Drosophila melanogaster Ampicillin PMID:21169635 Backbone Marker:Drosophila Gateway Collection; Vector Backbone:pAGW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:35 1
pRK5F-ALK5-HA(ecto)
 
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1+ mentions
RRID:Addgene_31720 ALK5 Homo sapiens Ampicillin This construct is beginning from a Signal Peptide of human TbRI/ALK5, followed by a HA tag, the extracellular cDNA sequence of human TbRI/ALK, and ended by a Flag tag. This vector to produces a secreted TbRI/ALK5 extracellular form, with two individual tags on each side. Backbone Size:4716; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:36 1
LZRS-Rfa
 
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1+ mentions
RRID:Addgene_31601 Chloramphenicol and Ampicillin PMID:15342384 Backbone Marker:Khavari Lab; Backbone Size:11100; Vector Backbone:LZRS-RfA; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:13:37 5
BoNT/A-LC
 
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1+ mentions
RRID:Addgene_31602 BoNT/A LC Ampicillin PMID:18940613 The author states that although the construct is from a toxic protein, it only contains one third of the biologically active protein,i.e., it is inactive and harmless per se, and it does not require BL4 level for its expression/manipulation. Toxic BoNT/A is made of two 'chains' (i.e. polypeptides) linked to each other by one disulfide bond. The light chain (LC) is 50KDa long, whereas the heavy chain (HC) is 100 KDa. The HC is necessary for the toxin to target and enter motorneurons. This construct is the LC of BoNTA, and it completely lacks the HC, so it is unable to intoxicate neurons. Protein sequence of BoNT/A fragment present in this plasmid (corresponds to YP_001386738.1) MSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFT Nucleotide sequence of BoNT/A fragment present in this plasmid based on Addgene's Sanger sequencing results: ATGAGTGGGTTAGAAGTAAGCTTTGAGGAACTTAGAACATTTGGGGGACATGATGCAAAGTTTATAGATAGTTTACAGGAAAACGAATTTCGTCTATATTATTATAATAAGTTTAAAGATATAGCAAGTACACTTAATAAAGCTAAATCAATAGTAGGTACTACTGCTTCATTACAGTATATGAAAAATGTTTTTAAAGAGAAATATCTCCTATCTGAAGATACATCTGGAAAATTTTCGGTAGATAAATTAAAATTTGATAAGTTATACAAAATGTTAACAGAGATTTACACAGAGGATAATTTTGTTAAGTTTTTTAAAGTACTTAACAGAAAAACATATTTGAATTTTGATAAAGCCGTATTTAAGATAAATATAGTACCTAAGGTAAATTACACAATATATGATGGATTTAATTTAAGAAATACAAATTTAGCAGCAAACTTTAATGGTCAAAATACAGAAATTAATAATATGAATTTTACTAAACTAAAAAATTTTACT Backbone Size:0; Vector Backbone:pBN3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:35 1
pDONR223-MCU
 
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1+ mentions
RRID:Addgene_31726 MCU Homo sapiens Spectinomycin PMID:21685886 *A3T aa residue substitution in MCU, which is not known to affect protein function Backbone Marker:Invitrogen; Backbone Size:2787; Vector Backbone:pDONR223; Vector Types:pDONR Gateway; Bacterial Resistance:Spectinomycin * 2026-08-15 01:13:39 1
pKin1A
 
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1+ mentions
RRID:Addgene_31607 kinesin-1A Mus musculus Kanamycin PMID:19812246 Backbone Marker:Clontech; Backbone Size:3940; Vector Backbone:pEGFP-C1 (EGFP removed); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:38 4

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