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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2 Resource Report Resource Website |
RRID:Addgene_205051 | GFP(-6)-H2_mCherry(-6)-L2 | Other | Ampicillin | PMID:38308651 | Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:28:54 | 0 | ||
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p663-UBC-MTS-miniTurbo-V5_IDG-K Resource Report Resource Website |
RRID:Addgene_214456 | MTS-miniTurbo-V5 | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:02 | 0 | ||
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p663-UBC-MTS-TurboID-V5_IDG-K Resource Report Resource Website |
RRID:Addgene_214457 | MTS-TurboID-V5 | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:03 | 0 | ||
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p663-UBC-PM-TurboID-V5_IDG-K Resource Report Resource Website 1+ mentions |
RRID:Addgene_214455 | PM-TurboID-V5 | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:02 | 1 | ||
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pUC-tRNAGln-BbsI-sU6-Kan Resource Report Resource Website |
RRID:Addgene_213016 | tRNAGln promoter, sU6 promoter, gRNA scaffolds | Other | Kanamycin | Backbone Size:2600; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:29:04 | 0 | |||
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pCfB6630 Resource Report Resource Website |
RRID:Addgene_212700 | gRNA targeting to the intergradtion site C3 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:05 | 0 | ||
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pCfB6638 Resource Report Resource Website |
RRID:Addgene_212698 | gRNA targeting to the intergradtion site E4 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:05 | 0 | ||
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pB-Neo-mtkeima Resource Report Resource Website |
RRID:Addgene_210338 | mtkeima | Other | Ampicillin | PMID:34048705 | Vector Backbone:pB-CAG-BGH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:06 | 0 | ||
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pCfB8866 Resource Report Resource Website |
RRID:Addgene_212703 | gRNA targeting to the intergradtion site F3 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:06 | 0 | ||
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pCfB8856 Resource Report Resource Website |
RRID:Addgene_212701 | gRNA targeting to the intergradtion site C3 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:06 | 0 | ||
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pCfB12719 Resource Report Resource Website |
RRID:Addgene_212702 | gRNA targeting to the intergradtion site F3 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:22 | 0 | ||
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MP6-SlugABEN-738 Resource Report Resource Website |
RRID:Addgene_214348 | dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE | other | Chloramphenicol | PMID:38052959 | Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:29:23 | 0 | ||
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pCfB11787 Resource Report Resource Website |
RRID:Addgene_212695 | gRNA targeting to the intergradtion site E2 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:22 | 0 | ||
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pGWB508-cTP-mCherry Resource Report Resource Website |
RRID:Addgene_213862 | Plastid transit peptide fused mCherry-UnaG | Other | Spectinomycin | PMID:36705704 | Vector Backbone:pGWB508; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:29:24 | 0 | ||
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pGWB602-po-mCerulean Resource Report Resource Website |
RRID:Addgene_213861 | Peroxisome transit peptide fused mCerulean | Other | Spectinomycin | PMID:36705704 | Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:29:24 | 0 | ||
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pCfB10769 Resource Report Resource Website |
RRID:Addgene_212694 | gRNA targeting to the intergradtion site E2 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:24 | 0 | ||
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MCP-YPet Resource Report Resource Website |
RRID:Addgene_215747 | YPet | Other | Ampicillin | PMID:38554702 | Vector Backbone:pHAGE-EFS-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:26 | 0 | ||
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pCfB11785 Resource Report Resource Website |
RRID:Addgene_212699 | gRNA targeting to the intergradtion site E4 | Other | Ampicillin | PMID:39260817 | Vector Backbone:custom backbone; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:26 | 0 | ||
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pSuperCRISPR-sgRNA-RL554-TTTA Resource Report Resource Website |
RRID:Addgene_215863 | sgRNA-RL554 with TTTA stretch | Other | Ampicillin | PMID:28177825 | Vector Backbone:pSuper; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:31 | 0 | ||
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pMBA324 Resource Report Resource Website |
RRID:Addgene_214741 | BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE | other | Kanamycin | PMID:38086386 | Please note: Plasmid contains N242S, R257H, and H289Y mutations in Rep101. These mutations are not known to affect plasmid function. | Vector Backbone:pSC101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:29:31 | 0 |
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