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Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Comments: This is a mutation of an in vitro site of phosphorylation.
Proper citation: RRID:Addgene_11219 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115241
Comments: This mutant begins at Rad 2nd ATG and contains a deletion of the calmodulin binding domain.
Proper citation: RRID:Addgene_11225 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115241
Comments: This mutant starts at the second methionine of Rad at residue 40. This mutant corresponds to the GST fusion construct of Rad used by Zhu and Moyers which begins at Rad 2nd ATG.
Proper citation: RRID:Addgene_11224 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Comments: This is a mutation of an in vitro site of phosphorylation.
Proper citation: RRID:Addgene_11221 Copy
Species: Homo sapiens
Genetic Insert: EYFP-YAP1
Vector Backbone Description: Backbone Size:7484; Vector Backbone:pLentilox3.7; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29909276
Comments: The human YAP1-2gamma isoform, which is 504 amino acids long is described in (Gaffney et al., 2012)
Proper citation: RRID:Addgene_112286 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Comments: This is a mutation of an in vitro site of phosphorylation.
Proper citation: RRID:Addgene_11222 Copy
Species: Homo sapiens
Genetic Insert: gRNAs targeting human miRNAs
Vector Backbone Description: Vector Backbone:modified pLX-sgRNA (Plasmid #50662); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29720387
Proper citation: RRID:Addgene_112200 Copy
Species: Homo sapiens
Genetic Insert: EYFP-YAP1
Vector Backbone Description: Backbone Size:7484; Vector Backbone:pLentilox3.7; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29909276
Comments: The human YAP1-2gamma isoform, which is 504 amino acids long is described in (Gaffney et al., 2012)
Proper citation: RRID:Addgene_112288 Copy
Species: Homo sapiens
Genetic Insert: EYFP-YAP1
Vector Backbone Description: Backbone Size:7484; Vector Backbone:pLentilox3.7; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29909276
Comments: The human YAP1-2gamma isoform, which is 504 amino acids long is described in (Gaffney et al., 2012)
Proper citation: RRID:Addgene_112289 Copy
Species: Homo sapiens
Genetic Insert: MAPT
Vector Backbone Description: Vector Backbone:pBlueScript-ISce1; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17897967
Proper citation: RRID:Addgene_112205 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115241
Comments: The C249 mutant contains a deletion of the calmodulin binding site.
Proper citation: RRID:Addgene_11228 Copy
Species: Homo sapiens
Genetic Insert: EYFP-YAP1
Vector Backbone Description: Backbone Size:7484; Vector Backbone:pLentilox3.7; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29909276
Comments: The human YAP1-2gamma isoform, which is 504 amino acids long is described in (Gaffney et al., 2012)
Proper citation: RRID:Addgene_112290 Copy
Species: Homo sapiens
Genetic Insert: p47phox
Vector Backbone Description: Vector Backbone:p87; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112250 Copy
Species: Homo sapiens
Genetic Insert: CRY2-mCherry-(GGGGS)3-hRGS2box
Vector Backbone Description: Backbone Size:7389; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29792810
Proper citation: RRID:Addgene_112257 Copy
Species: Homo sapiens
Genetic Insert: NtermRGS2-GFP-CIBN
Vector Backbone Description: Backbone Size:7389; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29792810
Proper citation: RRID:Addgene_112258 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:7583; Vector Backbone:pCMV histidinol; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10958681
Comments: pCMV human IRS-1 was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into pCMV human IRS-1. Select cells expressing plasmid with approximately 5mM histidinol (Sigma), depending on the cell type.
Proper citation: RRID:Addgene_11238 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10958681
Comments: IRS-1 pBABE was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into IRS-I pBABE.
(Kahn lab plasmid name = IRS-1 CAAX (Human) pBABE)
Proper citation: RRID:Addgene_11237 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Proper citation: RRID:Addgene_11232 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Proper citation: RRID:Addgene_11231 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4950; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9677319
Proper citation: RRID:Addgene_11234 Copy
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