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Species: Homo sapiens
Genetic Insert: CENP-A
Vector Backbone Description: Backbone Marker:Cheeseman/Desai (Addgene plasmid #44432); Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63072 Copy
Species: Homo sapiens
Genetic Insert: FANC-A, IMAGE clone 5725803
Vector Backbone Description: Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63076 Copy
Species: Homo sapiens
Genetic Insert: FAAP24, IMAGE clone 3659284
Vector Backbone Description: Vector Backbone:pIC242; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63077 Copy
Species: Rattus norvegicus
Genetic Insert: AP180
Vector Backbone Description: Vector Backbone:pQE-31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7814377
Proper citation: RRID:Addgene_63006 Copy
Species: Rattus norvegicus
Genetic Insert: Epsin 1 without ENTH domain
Vector Backbone Description: Backbone Marker:Qiagen; Vector Backbone:pQE-32; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11756460
Proper citation: RRID:Addgene_63008 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6156; Vector Backbone:pLenti7.3DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25887710
Proper citation: RRID:Addgene_63083 Copy
Species: Mus musculus
Genetic Insert: Mouse Nanog R147A Mutant
Vector Backbone Description: Backbone Marker:Shinya Yamanaka; Backbone Size:6378; Vector Backbone:pPyCAG-Nanog-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63002 Copy
Species: Homo sapiens
Genetic Insert: RBFOX2
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Contains their FAPY Nuclear localisation signal in Cterminus
Proper citation: RRID:Addgene_63086 Copy
Species: Synthetic
Genetic Insert: CTG REPEATS
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Addgene cannot verify or guarantee the presence of all 700 CTG repeats in this array
Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63087 Copy
Species: Mus musculus
Genetic Insert: Mouse Nanog Q144A Mutant
Vector Backbone Description: Backbone Marker:Shinya Yamanaka; Backbone Size:6378; Vector Backbone:pPyCAG-Nanog-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63000 Copy
Species: Rattus norvegicus
Genetic Insert: Endolyn
Vector Backbone Description: Backbone Marker:Girotti and Banting, PMID 9013339 ; Vector Backbone:delta pMEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10620506
Proper citation: RRID:Addgene_62962 Copy
Species: Rattus norvegicus
Genetic Insert: lgp120
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The 10 amino acid linker was designed using the sequence as described below but with different cloning sites (EcoRI and BamHI):
Patterson GH and Lippincott-Schwartz J. (2002) A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873-1877.
Examples of use:
Jahreiss L, Menzies FM, Rubinsztein DC. (2008) The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes. Traffic 9: 574-587.
Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC. (2011) Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex. J Cell Sci 124:469-482.
Ogunbayo OA, Zhu Y, Shen B, Agban E, Li J, Ma J, Zhu MX, Evans AM. (2015) Organelle-specific Subunit Interactions of the Vertebrate Two-pore Channel Family. J. Biol. Chem. 290: 1086-1095.
Proper citation: RRID:Addgene_62963 Copy
Species: Homo sapiens
Genetic Insert: CD63 molecule
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Examples of use:
Blagoveshchenskaya AD, Hannah MJ, Allen S, Cutler DF. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor. Mol Biol Cell 13:1582-1593.
Bampton ET, Goemans CG, Niranjan D, Mizushima N, Tolkovsky AM. (2005) The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes.
Autophagy 1: 23-36.
Harrison-Lavoie KJ, Michaux G, Hewlett L, Kaur J, Hannah MJ, Lui-Roberts WW, Norman KE, Cutler DF. (2006) P-selectin and CD63 use different mechanisms for delivery to Weibel-Palade bodies.
Traffic 7: 647-662.
Babich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T. (2008) Selective release of molecules from Weibel-Palade bodies during a lingering kiss. Blood 111: 5282-5290.
Babich V, Knipe L, Hewlett L, Meli A, Dempster J, Hannah MJ, Carter T. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
Proper citation: RRID:Addgene_62964 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster Or1a promoter
Vector Backbone Description: Backbone Marker:Leslie Vosshall Lab; Backbone Size:12000; Vector Backbone:pCasperAUG-Gal4-X; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16332533
Proper citation: RRID:Addgene_63019 Copy
Species: Mus musculus
Genetic Insert: BTRC
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25632159
Proper citation: RRID:Addgene_62977 Copy
Species: Mus musculus
Genetic Insert: BTRC
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25632159
Proper citation: RRID:Addgene_62978 Copy
Species: Homo sapiens
Genetic Insert: KLF4 partial sequence
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report-LUC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518959
Proper citation: RRID:Addgene_62979 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster Or33a promoter
Vector Backbone Description: Backbone Marker:Leslie Vosshall Lab; Backbone Size:12000; Vector Backbone:pCasperAUG-Gal4-X; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16139209
Proper citation: RRID:Addgene_63028 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster Or33b promoter
Vector Backbone Description: Backbone Marker:Leslie Vosshall Lab; Backbone Size:12000; Vector Backbone:pCasperAUG-Gal4-X; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16139209
Proper citation: RRID:Addgene_63029 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila melanogaster Or22c promoter
Vector Backbone Description: Backbone Marker:Leslie Vosshall Lab; Backbone Size:12000; Vector Backbone:pCasperAUG-Gal4-X; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16332533
Proper citation: RRID:Addgene_63024 Copy
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