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Species: Synthetic
Genetic Insert: VAMP7-T3'PAP
Vector Backbone Description: Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33602850
Comments: Additional insert: gIII/luxAB
Proper citation: RRID:Addgene_168002 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc with Y717E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_167959 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST TnsD
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168150 Copy
Species: Rattus norvegicus
Genetic Insert: Fz1-ER-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1, and GFP at the C terminus.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16815 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-2 cDNA was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The the insert was published by Wang et al., JBC 271: 4468 (1996), and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16813 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-2 cDNA, tagged with a single epitope in the third extracellular loop (at an introduced NheI site) was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16812 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST TnsC
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168149 Copy
Species: Anabaena variabilis
Genetic Insert: AvCAST right end (RE)
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168145 Copy
Species: Synthetic
Genetic Insert: ymVenus
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_168058 Copy
Species: Synthetic
Genetic Insert: ymScarletI
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Comments: Contains the linker region at the 5’ end of the FP, to use with the standard F5 forward primer (GGTGACGGTGCTGGTTTA).
Proper citation: RRID:Addgene_168055 Copy
Species: Synthetic
Genetic Insert: Blue light-inducible AraC dimers in Escherichia coli
Vector Backbone Description: Vector Backbone:pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33903769
Proper citation: RRID:Addgene_168051 Copy
Species: Homo sapiens
Genetic Insert: PGBD5
Vector Backbone Description: Backbone Marker:Genecopeia; Backbone Size:8721; Vector Backbone:pReceiver-Lv103; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504702
Proper citation: RRID:Addgene_168053 Copy
Species: Synthetic
Genetic Insert: mGreenLantern-T2A-mCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41919951
Proper citation: RRID:Addgene_168060 Copy
Species: Synthetic
Genetic Insert: ymTq2-yeVenus
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168076 Copy
Species: Synthetic
Genetic Insert: cyterm-mVenus
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_168079 Copy
Species: Synthetic
Genetic Insert: ystagRFP-T2A-mTq2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41919951
Proper citation: RRID:Addgene_168072 Copy
Species: Synthetic
Genetic Insert: mAvicFP1-T2A-mCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168073 Copy
Species: Synthetic
Genetic Insert: ymTq2-mAvicFP1
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41919951
Proper citation: RRID:Addgene_168075 Copy
Species: S.aureus
Genetic Insert: Cas9-P2A-mCherry
Vector Backbone Description: Backbone Marker:Addgene #40973; Vector Backbone:PBCAG-eGFP; Vector Types:Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288582
Comments: Puromycin selection disappears following Cre recombination.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.27.445896v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_168107 Copy
Species: Homo sapiens
Genetic Insert: HA-nEPEA-(EAAAK)3-OGA(544-706)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5352; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33686291
Proper citation: RRID:Addgene_168102 Copy
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