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Genetic Insert: SARS-CoV-2-NTD
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33091382
Proper citation: RRID:Addgene_160475 Copy
Species: Mus musculus
Genetic Insert: Id1 promoter region
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-basic vector (BV); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8628310
Proper citation: RRID:Addgene_16048 Copy
Species: Homo sapiens
Genetic Insert: beta1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2155708
Comments: Please note that the beta-1 integrin ORF has the following amino acid changes - C17G, M170T and N227S. Addgene is not aware of the impact of these mutations on the plasmid function.
Proper citation: RRID:Addgene_16042 Copy
Species: Synthetic
Genetic Insert: Opto-Cre-Vvd-2
Vector Backbone Description: Backbone Size:5118; Vector Backbone:pBbS5a; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961670
Proper citation: RRID:Addgene_160400 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32571767
Comments: Plasmid may be particularly prone to recombination; please screen multiple colonies before selecting a clone to perform downstream experiments
SFS in paper should be FSF
Proper citation: RRID:Addgene_160551 Copy
Species: Homo sapiens
Genetic Insert: hTRF1
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12169636
Comments: Karlseder et al., 2002, Science, 295, 2446-9
Proper citation: RRID:Addgene_16058 Copy
Species: Mus musculus
Genetic Insert: Multimerized E-box
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4200; Vector Backbone:pGL4.10 (luc2); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8628310
Comments: Contains three repeats of 5'-TTGGGAGGCAGCAGGTG-3'. p4RTK: has a minimal thymidine kinase promoter.
Proper citation: RRID:Addgene_16057 Copy
Species: Homo sapiens
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_16061 Copy
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33332762
Proper citation: RRID:Addgene_160675 Copy
Species: Synthetic
Genetic Insert: empty sgRNA (BsaI)
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33332762
Proper citation: RRID:Addgene_160666 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12169636
Comments: Dominate Negative form of TRF2
Proper citation: RRID:Addgene_16069 Copy
Species: Homo sapiens
Genetic Insert: hTRF2
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12169636
Proper citation: RRID:Addgene_16067 Copy
Species: Gallus gallus
Genetic Insert: beta-catenin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12123611
Comments: Phosphorylation-prevented mutant of beta catenin.
*Note: In addition to the described mutation, Addgene's quality control sequencing finds the following polymorphisms: L95R, S96A, G636A, and V651G.
Proper citation: RRID:Addgene_16072 Copy
Vector Backbone Description: Vector Backbone:pEG BacMam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25299155
Proper citation: RRID:Addgene_160683 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNA Polymerase II CTD
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468530
Proper citation: RRID:Addgene_160688 Copy
Species: Synthetic
Genetic Insert: SARS-CoV-2 NSP8
Vector Backbone Description: Backbone Size:3482; Vector Backbone:pcI-ts,ind+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33283177
Proper citation: RRID:Addgene_160656 Copy
Species: Homo sapiens
Genetic Insert: HA-nGFP-(EAAAK)4-OGT(4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32119511
Proper citation: RRID:Addgene_160739 Copy
Species: V. cholerae
Genetic Insert: VchCAST proteins and crRNA
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33230293
Comments: Previously referred to as INTEGRATE.
Proper citation: RRID:Addgene_160731 Copy
Species: V. cholerae
Genetic Insert: VchCAST donor DNA (Mini-Tn)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33230293
Comments: Previously referred to as INTEGRATE.
Proper citation: RRID:Addgene_160732 Copy
Species: E. coli
Genetic Insert: HSP33 cys
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5190; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16054089
Comments: The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.
Proper citation: RRID:Addgene_16076 Copy
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