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Vector Backbone Description: Backbone Size:5363; Vector Backbone:pBMTB-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16496398
Comments: Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.
Proper citation: RRID:Addgene_22825 Copy
Vector Backbone Description: Backbone Size:2936; Vector Backbone:pBT-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16496398
Proper citation: RRID:Addgene_22831 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: I-CreI homing endonuclease
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:8900; Vector Backbone:pSC101ts; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:19165721
Comments: Seligman, LM et al., 1997, Genetics, 147: 1653-1664
The plasmid can be propagated in 0.2% glucose to keep expression levels low and to guard against mutations.
Addgene's quality control sequence shows several sequence discrepancies with the available NCBI reference sequence for AraC and ICreI. Depositor tested this plasmid and found the plasmid can be used to cure cells of other plasmids that contain an I-CreI cut site, and the pCURE can be removed from cells via its temperature sensitive origin of replication. Hence, these mutations are not detrimental to the plasmid's function.
Proper citation: RRID:Addgene_23013 Copy
Species: E. coli
Genetic Insert: MG1655-tMMR
Vector Backbone Description: Vector Backbone:genomic; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24500200
Comments: For detailed use and MAGE, see:
1. Nyerges, Á. et al. Conditional DNA repair mutants enable highly precise genome engineering. Nucl. Acids Res. 42, e62–e62 (2014).
2. Gallagher, R. R., Li, Z., Lewis, A. O. & Isaacs, F. J. Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. Nat. Protocols 9, 2301–2316 (2014).
3. Bonde, M. T., Anderson, M. V., Wallin, A. I. N., Wang, H. H. & Sommer, M. O. A. MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucl. Acids Res. (2014). doi:10.1093/nar/gku428
Proper citation: RRID:Addgene_62392 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12778056
Comments: does not contain lacI/
ERG1, ERG8, MVD1: S. cerevisiae/
idi, ispA: E. Coli
Proper citation: RRID:Addgene_17817 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12778056
Comments: does not contain lacI
Proper citation: RRID:Addgene_17819 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NI6
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31001 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NI7
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31002 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NN3
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31009 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NN2
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31008 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NG6
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30990 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NG8
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30992 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NI1
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30996 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NI4
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30999 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat HD8
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30981 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat HD10
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30983 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NG1
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30985 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat HD1
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30974 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat HD2
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30975 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat HD4
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_30977 Copy
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