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Species: E. coli
Genetic Insert: [sd2] T7 RNAP-TCC linker-degron
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35379961
Proper citation: RRID:Addgene_179679 Copy
Species: Synthetic
Genetic Insert: [sd4U] UGI-TALE3-DddA-G1397-N wildtype
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35379961
Comments: araC
Proper citation: RRID:Addgene_179690 Copy
Species: Synthetic
Genetic Insert: [sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35379961
Comments: araC
Proper citation: RRID:Addgene_179691 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike T1027I
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180058 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike A570D
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180064 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike K417N
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180060 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike L241-L242-A243-
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180070 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 N S235F
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180071 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN7
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34854883
Comments: The mutant is the fusion of the SEPT9_i1-specific N-terminal sequence to SEPT9_i5.
Please note: Plasmid contains a 65bp insertion in the CloDF13 ori. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_180314 Copy
Species: Arabidopsis thaliana
Genetic Insert: RD21A genomic sequence including 2kb upstream of the ATG
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa, Sumie Ishiguro, Tetsuya Kimura; Backbone Size:8500; Vector Backbone:pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34877448
Proper citation: RRID:Addgene_181959 Copy
Species: Arabidopsis thaliana
Genetic Insert: SCPL35
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa, Sumie Ishiguro, Tetsuya Kimura; Backbone Size:8500; Vector Backbone:pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34877448
Proper citation: RRID:Addgene_181960 Copy
Species: Arabidopsis thaliana
Genetic Insert: ASPG1
Vector Backbone Description: Backbone Marker:Tsuyoshi NAKAGAWA, Shinya NAKAMURA, Katsunori TANAKA, Makoto KAWAMUKAI, Takamasa SUZUKI, Kenzo NAKAMURA, Tetsuya KIMUR; Backbone Size:12000; Vector Backbone:R4pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34877448
Proper citation: RRID:Addgene_181962 Copy
Species: Arabidopsis thaliana
Genetic Insert: ARA12
Vector Backbone Description: Backbone Marker:Tsuyoshi NAKAGAWA, Shinya NAKAMURA, Katsunori TANAKA, Makoto KAWAMUKAI, Takamasa SUZUKI, Kenzo NAKAMURA, Tetsuya KIMUR; Backbone Size:11998; Vector Backbone:R4pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34877448
Proper citation: RRID:Addgene_181961 Copy
Species: Bacteriophage lambda
Genetic Insert: Red-alpha, Red-beta, Gam
Vector Backbone Description: Backbone Size:0; Vector Backbone:F'; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16141338
Comments: pML300 carries the genes encoding the exo, beta, and gam recombination functions of bacteriophage lambda, as well as RecA, to render an E. coli strain recombination proficient. The lambda Red-alpha, Red-beta, and Gam proteins are under the rhaB promoter, induced by incubation with the sugar rhamnose. The bacterial strain is BW28705I (BUN21)/pML300 (recipient strain): lacIq rrnB3 delta-lacZ4787 hsdR514 delta-(araBAD)567 delta-(rhaBAD)568 galU95 delta-endA9::FRT delta-recA635::FRT umuC::ParaBAD-I-SceI-FRT + pML300(PrhaB-gam-beta-exo Ts(ori). Grow at 30'C in LB/Sp 50 ug/ml (spectinomycin resistant).
Proper citation: RRID:Addgene_11675 Copy
Species: Homo sapiens
Genetic Insert: Human MCM6
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25648893
Comments: Please note that mutation L657M in human MCM4 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_116951 Copy
Species: Other
Genetic Insert: ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117236 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-p (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117521 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-h (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287 ; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with Moclo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117520 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-p (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117522 Copy
Species: Streptococcus pyogenes
Genetic Insert: Sp-eCas9 1.1 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117529 Copy
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