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Species: Synthetic
Genetic Insert: Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
Vector Backbone Description: Backbone Marker:Terrence Lai (this study); Backbone Size:1878; Vector Backbone:colE1 origin and chloramphenicol resistance; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74091 Copy
Species: E. coli MG1655
Genetic Insert: lldR
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74095 Copy
Species: E. coli MG1655
Genetic Insert: Bba_K822000 (L-Lactate promoter - E. coli MG1655)
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74094 Copy
Species: Synthetic
Genetic Insert: TL9 RBS
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Comments: This plasmid contains the TL9 RBS upstream of GFP.
Proper citation: RRID:Addgene_74098 Copy
Species: Synthetic
Genetic Insert: Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
Vector Backbone Description: Backbone Marker:Terrence Lai (this study); Backbone Size:2024; Vector Backbone:p15A origin and chloramphenicol resistance; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74089 Copy
Species: E. coli
Genetic Insert: FtsZ-MEEVF
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_74126 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_74128 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtU6-1 promoter
Vector Backbone Description: Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26839579
Proper citation: RRID:Addgene_75405 Copy
Species: Synthetic
Genetic Insert: AD1 Sense
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25643173
Proper citation: RRID:Addgene_71207 Copy
Genetic Insert: pbuE Sense
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25643173
Proper citation: RRID:Addgene_71209 Copy
Vector Backbone Description: Backbone Size:4378; Vector Backbone:pGK12; Vector Types:Bacterial Expression, E. coli-gram positive bacteria shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11526048
Comments: In plasmid preparations from E. coli, a smaller deletion derivative of pTRK669 of about 1.7 kb may be noticeable. This smaller plasmid does not affect the functionality of pTRK669 in gram positive hosts and may not appear in those backgrounds. Addgene's quality control sequence shows the expected deletion from the smaller derivative of pTRK669.
Whole plasmid sequencing from the depositor showed deletion of the NheI site. Please see the provided digest result for reference.
Proper citation: RRID:Addgene_71313 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25769287
Proper citation: RRID:Addgene_71420 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25769287
Proper citation: RRID:Addgene_71421 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pSC101ts; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26141150
Comments: The plasmid curing system consists of an arabinose inducible promoter, PBAD to express a gRNA targeting the bla gene on the gRNA plasmid. When induced, the bla-targeting gRNA is expressed, leading Cas9 to cleave the gRNA plasmid and resulting in plasmid elimination.
Proper citation: RRID:Addgene_71538 Copy
Species: Other
Genetic Insert: loxP-hyg-cat-loxP
Vector Backbone Description: Backbone Marker:Jeffery Murry - Eric Rubin Lab; Vector Backbone:pJM1; Vector Types:Bacterial Expression, Source of loxP-hyg-cat- loxP for PCR amplification.; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25779316
Comments: This vector contains a hyg-cat cassette, flanked by loxP sites, that can be used for the construction of recombineeng substrates for M. smegmatis or M. tuberculosis. It contains a 1 bp change (relative to starting plasmid pJM1) at position 2008 (G to C) that eliminate a potential stop codon that could interfere with expression of downstream functions following excision of the cassette from the chromosome by Cre recombinase.
The hygromycin resistance gene encodes M13I and I111T variants compared to the NCBI reference [ADL66925.1]. These variants should not interfere with function.
Proper citation: RRID:Addgene_71487 Copy
Vector Backbone Description: Backbone Size:3865; Vector Backbone:pG1C; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71740 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30541104
Comments: Please note that the full plasmid sequence contains a few differences compared to GenBank ID: KM977569. These differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_71764 Copy
Genetic Insert: RhlR
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: Lower expression level for CinR. This plasmid provide receptor for pRhl and pCin tests.
Proper citation: RRID:Addgene_72298 Copy
Genetic Insert: RhlR
Vector Backbone Description: Vector Backbone:E. coli BW25113; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: This is an ancestor strain of CY027. The chloramphenicol resistance marker can be removed by Flp-FRT recombination to obtain CY027.
Proper citation: RRID:Addgene_72340 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: This is an ancestor strain of CY008. The chloramphenicol resistance marker can be removed by Flp-FRT recombination to obtain CY008.
Proper citation: RRID:Addgene_72337 Copy
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