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Genetic Insert: Lambda integrase attL1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52551 Copy
Genetic Insert: PhiC31o attB (full length)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21464299
Proper citation: RRID:Addgene_52552 Copy
Species: Synthetic
Genetic Insert: eYFP and Blasticigin resistance cassette
Vector Backbone Description: Backbone Marker:Thermor Fisher / Fermentas; Backbone Size:2972; Vector Backbone:pJet1.2; Vector Types:blunt cloning kit; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24748663
Proper citation: RRID:Addgene_52559 Copy
Species: Homo sapiens
Genetic Insert: Fast-GCaMP-EF05
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:7831; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23863808
Proper citation: RRID:Addgene_52558 Copy
Species: Homo sapiens
Genetic Insert: HMGCLL1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22865860
Proper citation: RRID:Addgene_52434 Copy
Species: Drosophila melanogaster
Genetic Insert: VGlut-Gal80
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBPGAL80Uw-6; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Addgene's quality control sequencing has found a number of discrepancies with the provided full plasmid sequence. These differences are not thought to affect plasmid function.
Proper citation: RRID:Addgene_52555 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT4
Vector Backbone Description: Backbone Marker:Bogan lab, unpublished; Backbone Size:5783; Vector Backbone:pB; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11416153
Comments: Plasmid is also called pB-G7. We use FACS to select infected cells that express GFP. There is a short linker between GLUT4 and GFP.
Proper citation: RRID:Addgene_52872 Copy
Species: Rattus norvegicus
Genetic Insert: Kir4.1
Vector Backbone Description: Vector Backbone:pZac 2.1; Vector Types:Mammalian Expression, AAV, Adeno-Associated Virus (); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24686787
Proper citation: RRID:Addgene_52874 Copy
Genetic Insert: hEµMAR-SFFV; eGFP
Vector Backbone Description: Backbone Size:6724; Vector Backbone:LeGO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23240650
Proper citation: RRID:Addgene_52633 Copy
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pSILENCER; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
Proper citation: RRID:Addgene_52675 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52716 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN4
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 493–511 from the start sequence of rat α-actinin-4 (the sense chain was 5’-gactatccaggagatgcagttcaagagactgcatctcctggatagtctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52679 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN1
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 476–494 from the start sequence of rat α-actinin-1 (the sense chain was 5’-gatctccaacgtcaacaagttcaagagacttgttgacgttggagatctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52676 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN2
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 224–242 from the start sequence of rat α-actinin-2 (the sense chain was 5’-tggcctgatggatcatgagttcaagagactcatgatccatcaggccatttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52677 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52717 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: tetO7-Pcyc1+LINKER
Vector Backbone Description: Vector Backbone:pFA6a-kanMX-tetO-Pcyc1 (41023); Vector Types:Yeast Expression, Promoter replacement; Bacterial Resistance:Ampicillin
Comments: This is the same as the "regular" tetO plasmids (41022, 41023, 41024, 41025, or 41026) with an additional LADYGAG linker. This allows amplification of tagging cassettes from the LINKER vectors using the same pair of primers. For the “regular” vectors the reverse primer is unique to each plasmid.
Proper citation: RRID:Addgene_52685 Copy
Species: Mus musculus
Genetic Insert: ABL1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23081945
Comments: Encodes isoform IV of mouse c-Abl, which is N-myristylated.
Proper citation: RRID:Addgene_52684 Copy
Species: Homo sapiens
Genetic Insert: HMGA2
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52727 Copy
Species: Homo sapiens
Genetic Insert: EGR1
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52724 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52721 Copy
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