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Genetic Insert: MKK7-EE
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2 3xFLAG; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28174228
Proper citation: RRID:Addgene_87777 Copy
Species: Homo sapiens
Genetic Insert: MKK4
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2 3xFLAG; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28174228
Proper citation: RRID:Addgene_87776 Copy
Genetic Insert: xylE
Vector Backbone Description: Backbone Marker:PMID: 9380741; Backbone Size:8550; Vector Backbone:pPR23; Vector Types:Allelic change mutagenesis; Bacterial Resistance:Gentamicin
Defining Citation: PMID:17074913
Comments: SacB is used for counter-selecton in Mycobacteria to force excision and loss of this plasmid to occur.
This plasmid has been used as an allelic exchange vector. We have used SpeI site for cloning of an insert (flanking regions of a target gene to be deleted), so other groups can also use SpeI (NotI and XbaI can be used as well).
Proper citation: RRID:Addgene_89632 Copy
Species: Pseudomonas - from plasmid pPH1JI
Genetic Insert: Gentamicin resistance gene
Vector Backbone Description: Backbone Marker:Natural; Backbone Size:60000; Vector Backbone:RP4; Vector Types:Helper BHR plasmid for mobilizing other plasmids carrying oriT; Bacterial Resistance:Gentamicin
Defining Citation: PMID:15212888
Comments: Depositor states that users can select for the chromosome with nalidixic acid.
Depositor states that growth on the respective antibiotic is sufficient to confirm plasmid integrity
Proper citation: RRID:Addgene_79813 Copy
Species: Synthetic
Genetic Insert: shRNA targeting UBR5
Vector Backbone Description: Backbone Marker:ATCC 10326362 ; Backbone Size:4232; Vector Backbone:pEN_TmiRc3 (pENTR1A-Gent); Vector Types:Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29742019
Comments: For use with pSLIK lentiviral system. Original publication:
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103
shRNA sequence obtained from the RNAi Codex:
A. Olson, N. Sheth, J. S. Lee, G. Hannon and R. Sachidanandam (2005) RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs. Nucleic Acids Research, 34, D153-D157
Proper citation: RRID:Addgene_81066 Copy
Species: Fungia coccina
Genetic Insert: mKO1
Vector Backbone Description: Vector Backbone:pUCP30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26937640
Proper citation: RRID:Addgene_78477 Copy
Species: Aequorea victoria
Genetic Insert: eCFP
Vector Backbone Description: Vector Backbone:pUCP30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26937640
Comments: Please note that Addgene identified the presence of an eCFP fluorophore rather than the stated eYFP.
Proper citation: RRID:Addgene_78476 Copy
Vector Backbone Description: Backbone Size:4922; Vector Backbone:pJQ200; Vector Types:Suicide plasmid; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: This is the most popular version of the pJQ200 series of vectors. The partial lacZ alpha gene does give blue/white selection, but colonies are only pale blue after 24 hours. Blue colour is clear after 36 hrs. or by using higher x-gal concentration.
Proper citation: RRID:Addgene_78497 Copy
Vector Backbone Description: Backbone Size:6500; Vector Backbone:P15A origin vector pACYC184Gm; Vector Types:Suicide vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: Base vector for suicide vectors pJQ200SK, pJQ200KS, pJQ200mp18, pJQ200mp19 which have been quite widely used for allelic replacement in rhizobia and other bacteria. sacB gene provides basis for selection of double recombinants.
Proper citation: RRID:Addgene_78496 Copy
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:suicide vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: Cosmid version of suicide vector for gene replacement. Same as pJQ200SK except for addition of cos site.
Proper citation: RRID:Addgene_78499 Copy
Species: Pan troglodytes
Genetic Insert: OSF-TRIM5alpha(chimpanzee)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79032 Copy
Species: Macaca mulatta
Genetic Insert: OSF-TRIM5alpha(rhesus)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79033 Copy
Species: Aotus trivirgatus
Genetic Insert: OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79036 Copy
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188604 Copy
Species: Other
Genetic Insert: PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV PE2; Vector Types:Mammalian Expression, CRISPR, Recombinant baculovirus production, CRE-acceptor; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206274 Copy
Genetic Insert: dddI
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32641830
Comments: Genotype: F– φ80lacZΔM15 Δ(lacZYAargF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1, with chromosomally integrated dddI
PCR verification primers (expected product: ~450 bp): dddI-F: 5'-ATTCGAGCTCACGGGAGGAA-3' and dddI-R: 5'-CCTGCAGGTCGACCTCATTT-3'
Proper citation: RRID:Addgene_179631 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold - Gm resistance cassette - incP oriT
Vector Backbone Description: Backbone Size:8398; Vector Backbone:pS448∙CsR; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_197860 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH2 Terminator
Vector Backbone Description: Backbone Size:2383; Vector Backbone:pUCGE; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37575400
Proper citation: RRID:Addgene_200713 Copy
Species: Synthetic
Genetic Insert: GAL4BD-VP16
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDON207; Vector Types:Plant Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37323061
Comments: Encodes a chimeric transcription factor ORF consisting of the yeast GAL4 DNA binding domain and HSV VP16 activation domain. No promoter.
Proper citation: RRID:Addgene_201230 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:1892; Vector Backbone:BASIC_SEVA_66.11; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36381610
Proper citation: RRID:Addgene_194213 Copy
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