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CP2-TCC
 
Resource Report
Resource Website 		RRID:Addgene_179679 [sd2] T7 RNAP-TCC linker-degron E. coli Spectinomycin PMID:35379961 Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:23 0
pBM13h-sd4U
 
Resource Report
Resource Website 		RRID:Addgene_179690 [sd4U] UGI-TALE3-DddA-G1397-N wildtype Synthetic Spectinomycin PMID:35379961 araC Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:23 0
pBM13-sd4U
 
Resource Report
Resource Website 		RRID:Addgene_179691 [sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I) Synthetic Spectinomycin PMID:35379961 araC Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin Q1310R, S1330I, T1380I 2026-08-15 01:11:23 0
pDON223 Spike T1027I
 
Resource Report
Resource Website 		RRID:Addgene_180058 SARS-CoV-2 Spike T1027I SARS-CoV-2 Spectinomycin PMID:34709727 Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin Spike T1027I 2026-08-15 01:11:24 0
pDON223 Spike A570D
 
Resource Report
Resource Website 		RRID:Addgene_180064 SARS-CoV-2 Spike A570D SARS-CoV-2 Spectinomycin PMID:34709727 Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin Spike A570D 2026-08-15 01:11:24 0
pDON223 Spike K417N
 
Resource Report
Resource Website 		RRID:Addgene_180060 SARS-CoV-2 Spike K417N SARS-CoV-2 Spectinomycin PMID:34709727 Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin Spike K417N 2026-08-15 01:11:24 0
pDON223 Spike L241-L242-A243-
 
Resource Report
Resource Website 		RRID:Addgene_180070 SARS-CoV-2 Spike L241-L242-A243- SARS-CoV-2 Spectinomycin PMID:34709727 Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin Spike L241-L242-A243- 2026-08-15 01:11:26 0
pDON223 N S235F
 
Resource Report
Resource Website 		RRID:Addgene_180071 SARS-CoV-2 N S235F SARS-CoV-2 Spectinomycin PMID:34709727 Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin N S235F 2026-08-15 01:11:24 0
pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep
 
Resource Report
Resource Website 		RRID:Addgene_180314 SEPTIN7 Homo sapiens Spectinomycin PMID:34854883 The mutant is the fusion of the SEPT9_i1-specific N-terminal sequence to SEPT9_i5. Please note: Plasmid contains a 65bp insertion in the CloDF13 ori. This insertion is not known to affect plasmid function. Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:25 0
gRD21A-mRFP
 
Resource Report
Resource Website 		RRID:Addgene_181959 RD21A genomic sequence including 2kb upstream of the ATG Arabidopsis thaliana Spectinomycin PMID:34877448 Backbone Marker:Tsuyoshi Nakagawa, Sumie Ishiguro, Tetsuya Kimura; Backbone Size:8500; Vector Backbone:pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:29 0
gSCPL35-mRFP
 
Resource Report
Resource Website 		RRID:Addgene_181960 SCPL35 Arabidopsis thaliana Spectinomycin PMID:34877448 Backbone Marker:Tsuyoshi Nakagawa, Sumie Ishiguro, Tetsuya Kimura; Backbone Size:8500; Vector Backbone:pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:29 0
cASPG1-mRFP
 
Resource Report
Resource Website 		RRID:Addgene_181962 ASPG1 Arabidopsis thaliana Spectinomycin PMID:34877448 Backbone Marker:Tsuyoshi NAKAGAWA, Shinya NAKAMURA, Katsunori TANAKA, Makoto KAWAMUKAI, Takamasa SUZUKI, Kenzo NAKAMURA, Tetsuya KIMUR; Backbone Size:12000; Vector Backbone:R4pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:29 0
cARA12-mRFP
 
Resource Report
Resource Website 		RRID:Addgene_181961 ARA12 Arabidopsis thaliana Spectinomycin PMID:34877448 Backbone Marker:Tsuyoshi NAKAGAWA, Shinya NAKAMURA, Katsunori TANAKA, Makoto KAWAMUKAI, Takamasa SUZUKI, Kenzo NAKAMURA, Tetsuya KIMUR; Backbone Size:11998; Vector Backbone:R4pGWB553; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:29 0
BUN21/pML300 (recipient strain)
 
Resource Report
Resource Website 		RRID:Addgene_11675 Red-alpha, Red-beta, Gam Bacteriophage lambda Spectinomycin PMID:16141338 pML300 carries the genes encoding the exo, beta, and gam recombination functions of bacteriophage lambda, as well as RecA, to render an E. coli strain recombination proficient. The lambda Red-alpha, Red-beta, and Gam proteins are under the rhaB promoter, induced by incubation with the sugar rhamnose. The bacterial strain is BW28705I (BUN21)/pML300 (recipient strain): lacIq rrnB3 delta-lacZ4787 hsdR514 delta-(araBAD)567 delta-(rhaBAD)568 galU95 delta-endA9::FRT delta-recA635::FRT umuC::ParaBAD-I-SceI-FRT + pML300(PrhaB-gam-beta-exo Ts(ori). Grow at 30'C in LB/Sp 50 ug/ml (spectinomycin resistant). Backbone Size:0; Vector Backbone:F'; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:47 0
pCDF-MCM4/6
 
Resource Report
Resource Website 		RRID:Addgene_116951 Human MCM6 Homo sapiens Spectinomycin PMID:25648893 Please note that mutation L657M in human MCM4 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function. Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:49 0
pBECKP-spe
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117236 ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase Other Spectinomycin PMID:30217854 Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:52 1
pEPOR0SP0013
 
Resource Report
Resource Website 		RRID:Addgene_117521 SpCas9-p (with stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pICSL90005
 
Resource Report
Resource Website 		RRID:Addgene_117520 SpCas9-h (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with Moclo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287 ; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pEPOR0SP0009
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117522 SpCas9-p (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 1
pEPOR0SP0019
 
Resource Report
Resource Website 		RRID:Addgene_117529 Sp-eCas9 1.1 (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K848A, K1003A, R1060A 2026-08-15 01:02:54 0

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