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Plasmids are provided by Addgene and DGRC.

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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBMTB-4
 
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RRID:Addgene_22825 Tetracycline PMID:16496398 Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. Backbone Size:5363; Vector Backbone:pBMTB-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:12:16 0
pBT-4
 
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1+ mentions
RRID:Addgene_22831 Tetracycline PMID:16496398 Backbone Size:2936; Vector Backbone:pBT-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:12:15 1
pCURE
 
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RRID:Addgene_23013 I-CreI homing endonuclease Chlamydomonas reinhardtii Tetracycline PMID:19165721 Seligman, LM et al., 1997, Genetics, 147: 1653-1664 The plasmid can be propagated in 0.2% glucose to keep expression levels low and to guard against mutations. Addgene's quality control sequence shows several sequence discrepancies with the available NCBI reference sequence for AraC and ICreI. Depositor tested this plasmid and found the plasmid can be used to cure cells of other plasmids that contain an I-CreI cut site, and the pCURE can be removed from cells via its temperature sensitive origin of replication. Hence, these mutations are not detrimental to the plasmid's function. Backbone Marker:N/A; Backbone Size:8900; Vector Backbone:pSC101ts; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline I-CreI expression is controlled by the arabinose promoter. 2026-08-15 01:12:17 0
MG1655-tMMR
 
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RRID:Addgene_62392 MG1655-tMMR E. coli Tetracycline PMID:24500200 For detailed use and MAGE, see: 1. Nyerges, Á. et al. Conditional DNA repair mutants enable highly precise genome engineering. Nucl. Acids Res. 42, e62–e62 (2014). 2. Gallagher, R. R., Li, Z., Lewis, A. O. & Isaacs, F. J. Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. Nat. Protocols 9, 2301–2316 (2014). 3. Bonde, M. T., Anderson, M. V., Wallin, A. I. N., Wang, H. H. & Sommer, M. O. A. MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering. Nucl. Acids Res. (2014). doi:10.1093/nar/gku428 Vector Backbone:genomic; Vector Types:; Bacterial Resistance:Tetracycline mutS(A134V) mutL(G62S); nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} 2026-08-15 01:17:52 0
pMBIS
 
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1+ mentions
RRID:Addgene_17817 mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase Saccharomyces cerevisiae Tetracycline PMID:12778056 does not contain lacI/ ERG1, ERG8, MVD1: S. cerevisiae/ idi, ispA: E. Coli Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:11:15 6
pMevB
 
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1+ mentions
RRID:Addgene_17819 mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse Saccharomyces cerevisiae Tetracycline PMID:12778056 does not contain lacI Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:11:15 1
pNI6
 
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RRID:Addgene_31001 repeat NI6 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:27 0
pNI7
 
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RRID:Addgene_31002 repeat NI7 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:30 0
pNN3
 
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RRID:Addgene_31009 repeat NN3 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:31 0
pNN2
 
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RRID:Addgene_31008 repeat NN2 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:31 0
pNG6
 
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RRID:Addgene_30990 repeat NG6 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:30 0
pNG8
 
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RRID:Addgene_30992 repeat NG8 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:27 0
pNI1
 
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RRID:Addgene_30996 repeat NI1 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:30 0
pNI4
 
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RRID:Addgene_30999 repeat NI4 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:30 0
pHD8
 
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RRID:Addgene_30981 repeat HD8 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:30 0
pHD10
 
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RRID:Addgene_30983 repeat HD10 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:27 0
pNG1
 
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1+ mentions
RRID:Addgene_30985 repeat NG1 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:30 1
pHD1
 
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RRID:Addgene_30974 repeat HD1 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:27 0
pHD2
 
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RRID:Addgene_30975 repeat HD2 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:30 0
pHD4
 
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RRID:Addgene_30977 repeat HD4 Xanthamonas oryzae Tetracycline PMID:21493687 Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:13:27 0

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