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Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99891 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99894 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99893 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99885 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99884 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99887 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99889 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29197638
Proper citation: RRID:Addgene_99888 Copy
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Backbone Size:3600; Vector Backbone:pHD1; Vector Types:CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:35596077
Proper citation: RRID:Addgene_179216 Copy
Vector Backbone Description: Vector Backbone:pYPQ132-STU-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36127876
Proper citation: RRID:Addgene_189783 Copy
Vector Backbone Description: Vector Backbone:pYPQ134-STU-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36127876
Proper citation: RRID:Addgene_189785 Copy
Species: Gallus gallus
Genetic Insert: chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
Vector Backbone Description: Backbone Size:3055; Vector Backbone:pNG3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29386245
Comments: Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417.
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202
Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
Proper citation: RRID:Addgene_92394 Copy
Species: Synthetic
Genetic Insert: sgRNA towards phaZ in Pseudomonas putida
Vector Backbone Description: Vector Backbone:pBBR1k-GFP; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34175462
Proper citation: RRID:Addgene_169874 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25853840
Comments: Precursor strain: ElectroMAX DH10B E. coli cells
Landing pad cassette at at the aptI/gidB locus includes in this order: I-SceI restriction site - Landing Pad 1 (LP1) sequence - tetA gene - Landing Pad 2 (LP2) sequence - I-SceI restriction site.
This strain was made based on the technology described in the Kulman and Cox 2010 Nucleic Acids Res. paper: Site-specific chromosomal integration of large synthetic constructs. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847246/
Proper citation: RRID:Addgene_83036 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL1329 (salA) containing all of RL1328 (salR)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83785 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83784 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL0996 containing all of RL0997
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83782 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: promoter of RL1860
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83778 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: upstream region of RL2720 (rbsC)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83779 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL0990 (matA) containing all of RL0989 (matR)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83790 Copy
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