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Species: Danio rerio
Genetic Insert: ZFCNGA5
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:3022; Vector Backbone:pGEMHE; Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16887101
Comments: Note: This plasmid has an S132P amino acid residue substitution introduced via PCR, but this residue change is not thought to have any effect on ZFCNGA5 physiology or function
Proper citation: RRID:Addgene_69861 Copy
Species: Danio rerio
Genetic Insert: mfap4 promoter
Vector Backbone Description: Backbone Size:2765; Vector Backbone:p5E-MCS; Vector Types:Gateway 5' Element; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26445458
Comments: Please note: Addgene's quality control sequencing has identified a number of single-nucleotide discrepancies, compared to the NCBI Genbank reference sequence for the Zebrafish mfap4 promoter. The depositing laboratory is aware of these differences, and states that they are polymorphisms. This plasmid accurately reflects the construct as it was used in the associated publication.
Proper citation: RRID:Addgene_70052 Copy
Species: Danio rerio
Genetic Insert: zebrafish PSD95
Vector Backbone Description: Backbone Marker:clontech/invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14758365
Comments: The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_74315 Copy
Species: Danio rerio
Genetic Insert: zebrafish PSD95
Vector Backbone Description: Backbone Marker:Clontech/Invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14758365
Comments: The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_74314 Copy
Species: Danio rerio
Genetic Insert: synaptophysin
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16571769
Proper citation: RRID:Addgene_74317 Copy
Species: Danio rerio
Genetic Insert: cmlc2::mCherry
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27003937
Proper citation: RRID:Addgene_74624 Copy
Species: Danio rerio
Genetic Insert: FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Cre/Lox, Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27003937
Proper citation: RRID:Addgene_74625 Copy
Species: Danio rerio
Genetic Insert: macrophage expressed 1, tandem duplicate 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2763; Vector Backbone:pDONRP2R-P3; Vector Types:5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75023 Copy
Species: Danio rerio
Genetic Insert: ELAV like neuron-specific RNA binding protein 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2763; Vector Backbone:pDONRP2R-P3; Vector Types:5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75025 Copy
Species: Danio rerio
Genetic Insert: Nanog
Vector Backbone Description: Vector Backbone:pPB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22028025
Proper citation: RRID:Addgene_74910 Copy
Species: Danio rerio
Genetic Insert: elavl3 enhancer
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Gal80 intersectional regulation of cell-type specific expression in vertebrates. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. PubMed PMID:21905164; PubMed Central PMCID: PMC3178006.
Proper citation: RRID:Addgene_72640 Copy
Species: Danio rerio
Genetic Insert: Fv2E-flt4-2A-tdTomato
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27621059
Proper citation: RRID:Addgene_67698 Copy
Species: Danio rerio
Genetic Insert: zebrafish Chk1
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25558827
Proper citation: RRID:Addgene_67757 Copy
Species: Danio rerio
Genetic Insert: arrSa
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3973; Vector Backbone:pcRII-TOPO; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21299656
Proper citation: RRID:Addgene_78169 Copy
Species: Danio rerio
Genetic Insert: -3mnx1
Vector Backbone Description: Backbone Marker:Chien Lab; Backbone Size:4097; Vector Backbone:pTol2pA2; Vector Types:Zebrafish motor neuron inducible expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27631880
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_78251 Copy
Species: Danio rerio
Genetic Insert: beta actin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7903; Vector Backbone:pDestTol2pA; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934216
Proper citation: RRID:Addgene_162069 Copy
Species: Danio rerio
Genetic Insert: cytosolic phospholipase a2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27203112
Proper citation: RRID:Addgene_162566 Copy
Species: Danio rerio
Genetic Insert: cytosolic phospholipase a2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27203112
Proper citation: RRID:Addgene_162568 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting NFATc1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NFATc1(see below). Please note that this plasmid does NOT contain the NFATc1 sequence.
Users must order the complementary plasmid NFATc1_L (OZ543) [Addgene plasmid 28068] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGGA-3' in a bacterial two hybrid assay, and resulted in 7.97 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_28069 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting Encephalopsin (Opn3)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Encephalopsin (Opn3)(see below). Please note that this plasmid does NOT contain the Encephalopsin (Opn3) sequence.
Users must order the complementary plasmid Encephalopsin (Opn3)_R (OZ542) [Addgene plasmid 28067] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-TGGGATGGA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_28066 Copy
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