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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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pGEMHEZFCNGA5 Resource Report Resource Website |
RRID:Addgene_69861 | ZFCNGA5 | Danio rerio | Ampicillin | PMID:16887101 | Note: This plasmid has an S132P amino acid residue substitution introduced via PCR, but this residue change is not thought to have any effect on ZFCNGA5 physiology or function | Backbone Marker:unknown; Backbone Size:3022; Vector Backbone:pGEMHE; Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:19:01 | 0 |
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p5E-mfap4 Resource Report Resource Website |
RRID:Addgene_70052 | mfap4 promoter | Danio rerio | Kanamycin | PMID:26445458 | Please note: Addgene's quality control sequencing has identified a number of single-nucleotide discrepancies, compared to the NCBI Genbank reference sequence for the Zebrafish mfap4 promoter. The depositing laboratory is aware of these differences, and states that they are polymorphisms. This plasmid accurately reflects the construct as it was used in the associated publication. | Backbone Size:2765; Vector Backbone:p5E-MCS; Vector Types:Gateway 5' Element; Bacterial Resistance:Kanamycin | 2026-08-15 01:19:02 | 0 | |
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14UAS PSD95:GFP 5UAS DSRedExpress Resource Report Resource Website 1+ mentions |
RRID:Addgene_74315 | zebrafish PSD95 | Danio rerio | Kanamycin | PMID:14758365 | The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function. | Backbone Marker:clontech/invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin | Y599H and I793S (please see depositor comment below) | 2026-08-15 01:19:36 | 2 |
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14UAS zfPSD95:GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_74314 | zebrafish PSD95 | Danio rerio | Kanamycin | PMID:14758365 | The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function. | Backbone Marker:Clontech/Invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin | Y599H and I793S (please see depositor comment below) | 2026-08-15 01:19:36 | 2 |
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5UAS zfSynaptophysin:GFP-5UAS DsRedExpress Resource Report Resource Website 1+ mentions |
RRID:Addgene_74317 | synaptophysin | Danio rerio | Kanamycin | PMID:16571769 | Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:19:36 | 1 | ||
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pKHR7 Resource Report Resource Website |
RRID:Addgene_74624 | cmlc2::mCherry | Danio rerio | Ampicillin | PMID:27003937 | Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:38 | 0 | ||
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pKHR8 Resource Report Resource Website 1+ mentions |
RRID:Addgene_74625 | FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette | Danio rerio | Ampicillin | PMID:27003937 | Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Cre/Lox, Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:38 | 2 | ||
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p5E-mpeg1.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_75023 | macrophage expressed 1, tandem duplicate 1 | Danio rerio | Kanamycin | PMID:27631880 | Backbone Marker:Invitrogen; Backbone Size:2763; Vector Backbone:pDONRP2R-P3; Vector Types:5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin | 2026-08-15 01:19:42 | 2 | ||
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p5E-elavl3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_75025 | ELAV like neuron-specific RNA binding protein 3 | Danio rerio | Kanamycin | PMID:27631880 | Backbone Marker:Invitrogen; Backbone Size:2763; Vector Backbone:pDONRP2R-P3; Vector Types:5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin | 2026-08-15 01:19:42 | 1 | ||
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pPB-CAG-zNanogHD-pgk-hph Resource Report Resource Website |
RRID:Addgene_74910 | Nanog | Danio rerio | Ampicillin | PMID:22028025 | Vector Backbone:pPB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:19:41 | 0 | ||
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p5E-elavl3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_72640 | elavl3 enhancer | Danio rerio | Kanamycin | Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Gal80 intersectional regulation of cell-type specific expression in vertebrates. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. PubMed PMID:21905164; PubMed Central PMCID: PMC3178006. | Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:19:21 | 3 | ||
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pCS-Fv2Eflt4um200-2a-tdTomato Resource Report Resource Website |
RRID:Addgene_67698 | Fv2E-flt4-2A-tdTomato | Danio rerio | Ampicillin | PMID:27621059 | Vector Backbone:pCSDest2; Vector Types:; Bacterial Resistance:Ampicillin | um200 | 2026-08-15 01:18:41 | 0 | |
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pMZ039 Resource Report Resource Website |
RRID:Addgene_67757 | zebrafish Chk1 | Danio rerio | Ampicillin | PMID:25558827 | Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin | only encodes N-term kinase domain (amino acids 1-99) | 2026-08-15 01:18:38 | 0 | |
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arrSa Resource Report Resource Website |
RRID:Addgene_78169 | arrSa | Danio rerio | Ampicillin | PMID:21299656 | Backbone Marker:invitrogen; Backbone Size:3973; Vector Backbone:pcRII-TOPO; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:22 | 0 | ||
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pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS Resource Report Resource Website |
RRID:Addgene_78251 | -3mnx1 | Danio rerio | Ampicillin | PMID:27631880 | Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. | Backbone Marker:Chien Lab; Backbone Size:4097; Vector Backbone:pTol2pA2; Vector Types:Zebrafish motor neuron inducible expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:22 | 0 | |
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LR (bact: HyPer) Resource Report Resource Website |
RRID:Addgene_162069 | beta actin 2 | Danio rerio | Ampicillin | PMID:23934216 | Backbone Marker:Invitrogen; Backbone Size:7903; Vector Backbone:pDestTol2pA; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin | 2026-08-15 01:08:59 | 0 | ||
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pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1 Resource Report Resource Website |
RRID:Addgene_162566 | cytosolic phospholipase a2 | Danio rerio | Ampicillin | PMID:27203112 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:03 | 0 | ||
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pSB-CMV-MCS-Puro cPLA2-EGFP Resource Report Resource Website |
RRID:Addgene_162568 | cytosolic phospholipase a2 | Danio rerio | Ampicillin | PMID:27203112 | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:03 | 0 | ||
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NFATc1_R (OZ544) Resource Report Resource Website |
RRID:Addgene_28069 | Zinc finger array targeting NFATc1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NFATc1(see below). Please note that this plasmid does NOT contain the NFATc1 sequence. Users must order the complementary plasmid NFATc1_L (OZ543) [Addgene plasmid 28068] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGGA-3' in a bacterial two hybrid assay, and resulted in 7.97 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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Encephalopsin (Opn3)_L (OZ541) Resource Report Resource Website |
RRID:Addgene_28066 | Zinc finger array targeting Encephalopsin (Opn3) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Encephalopsin (Opn3)(see below). Please note that this plasmid does NOT contain the Encephalopsin (Opn3) sequence. Users must order the complementary plasmid Encephalopsin (Opn3)_R (OZ542) [Addgene plasmid 28067] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGGGATGGA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 |
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