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Species: Gallus gallus
Genetic Insert: DCTN1
Vector Backbone Description: Backbone Marker:Nature Technology Corporation; Backbone Size:4241; Vector Backbone:pVEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10525538
Proper citation: RRID:Addgene_74170 Copy
Species: Gallus gallus
Genetic Insert: FAK
Vector Backbone Description: Vector Backbone:pBS-KS-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1594631
Proper citation: RRID:Addgene_74478 Copy
Species: Gallus gallus
Genetic Insert: rASAP
Vector Backbone Description: Vector Backbone:pCDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34620975
Proper citation: RRID:Addgene_178205 Copy
Species: Gallus gallus
Genetic Insert: SEMA4D
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:2900; Vector Backbone:pBLUESCRIPT (SK); Vector Types:pBlue for ISH; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29568 Copy
Species: Gallus gallus
Genetic Insert: 2XDR4
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Comments: Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_32615 Copy
Species: Gallus gallus
Genetic Insert: rME
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32613 Copy
Species: Gallus gallus
Genetic Insert: rGH-ABC
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Comments: Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_32618 Copy
Species: Gallus gallus
Genetic Insert: TRalpha shRNA #1
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32619 Copy
Species: Gallus gallus
Genetic Insert: rGH-PAL
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32616 Copy
Species: Gallus gallus
Genetic Insert: GAPDH shRNA #1
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32621 Copy
Species: Gallus gallus
Genetic Insert: GAPDH shRNA #3
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32623 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Vector Backbone:pBlueRIP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9064352
Comments: ApaI/NotI removes a 3.26 kb RIP/cOVA/polyA insert
Proper citation: RRID:Addgene_69596 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Vector Backbone:pBlueRIP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9064352
Comments: ApaI/NotI removes a 2.3 kb RIP/cOVA/polyA insert. The rat insulin promoter is located at bp# 2794-2083
Proper citation: RRID:Addgene_69595 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Vector Backbone:pBlueRIP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939860
Proper citation: RRID:Addgene_69597 Copy
Species: Gallus gallus
Genetic Insert: Cx45
Vector Backbone Description: Backbone Marker:Fuhlbrigge, et al 1998, PMID 3261013; Vector Backbone:pSFFV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1382884
Comments: This plasmid was provided by Joseph Stains (University of Maryland School of Medicine), who received it from Thomas Steinberg (Washington University).
Proper citation: RRID:Addgene_69808 Copy
Species: Gallus gallus
Genetic Insert: Fzd7
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAG; Vector Types:Chicken expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25617437
Proper citation: RRID:Addgene_66106 Copy
Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type E
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613797
Proper citation: RRID:Addgene_67509 Copy
Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type B2
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22114284
Proper citation: RRID:Addgene_67513 Copy
Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type B
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20954846
Proper citation: RRID:Addgene_67523 Copy
Species: Gallus gallus
Genetic Insert: actin prom-dsRed-scramble shRNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25706476
Comments: To express shRNAs, we used the pPRIME system. This generates
micro-RNA-30 (mir30)-derived shRNAs and co-expression of a marker protein, such as dsRED, from the same transcript. The shRNA hairpin sequences were placed into the mir30 site of pPRIME-cmv-dsRed-FF3 and then cloned into the AAV vector (Zhang et al 2015 Nat Neurosci),
SbfI(or PstI) + PacI will retrieve the 2.1kb insert (dsRed-scramble shRNA-chloramphenicol) and the 5.1kb AAV-CBA backbone, which is ampicillin resistant. The SbfI site in the middle of dsRed has been silenced. The construct can be linearised with XhoI.
Proper citation: RRID:Addgene_67879 Copy
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