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Species: Mus musculus
Genetic Insert: dnPKA
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8812120
Comments: Insert: dominant negative Protein Kinase A-bright green flourescent protein fusion protein.
To make sense RNA, for expression, cut with Not1 and transcribe with SP6.
Plasmid History:
Constructed by Anne Ungar in 1996.
Citation: Ungar and Moon (1996)
Developmental Biology 178:186-191.
Proper citation: RRID:Addgene_16716 Copy
Species: Mus musculus
Genetic Insert: Casein Kinase II beta-pCS2P+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: Subcloned from XE 164 into Stu/Xba sites of CS2P+
Linearize with ASP718 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_16729 Copy
Species: Mus musculus
Genetic Insert: Mouse Naked-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: Subcloned Keith Wharton pCDNA3 insert into Xba (blunted) and Xho sites of CS2P+
Linearize with Sac2 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Addgene QC found an E431K mutation that is not known to affect function.
Proper citation: RRID:Addgene_16728 Copy
Species: Mus musculus
Genetic Insert: Ribo-GCaMP8m
Vector Backbone Description: Backbone Size:2868; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_167574 Copy
Species: Mus musculus
Genetic Insert: gRNA targeting Zmat3
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_167855 Copy
Species: Mus musculus
Genetic Insert: sgRNA targeting Zmat3
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_167853 Copy
Species: Mus musculus
Genetic Insert: mFZ6-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: The clone mFZ4/pRK5 from Nathans lab (accession # U43319), was used as template to PCR amplify just the coding region as an EcoR1 insert. The 3' stop was kept.
Constructed by: Rebecca Bates/Randall Moon.
Notes for Use:
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16802 Copy
Species: Mus musculus
Genetic Insert: FZ7
Vector Backbone Description: Backbone Size:4095; Vector Backbone:PCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: 3 Kb 5' EcoRI - 3' XbaI insert of mFZ7/pRK5 (Nathans), with engineered optimal kozak sequence.
Plasmid History:
Species of Sequence Origin: Xenopus.
Constructed by: Rebecca Bates/Randall Moon.
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16801 Copy
Species: Mus musculus
Genetic Insert: Hrasls3
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-IPU-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_167987 Copy
Species: Mus musculus
Genetic Insert: mGSDMD(1-276)
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4750; Vector Backbone:mCherry2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34540558
Proper citation: RRID:Addgene_168266 Copy
Species: Mus musculus
Genetic Insert: Bin1
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32170110
Proper citation: RRID:Addgene_168282 Copy
Species: Mus musculus
Genetic Insert: CD47
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6079; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34197341
Proper citation: RRID:Addgene_168479 Copy
Species: Mus musculus
Genetic Insert: Wnt-1-myc
Vector Backbone Description: Backbone Size:2746; Vector Backbone:pGEM4; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2673541
Comments: Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.
Species of Sequence Origin: mouse.
Notes for Use: Linearize with Eco R1 and transcribe with T7 to make RNA.
Reference: This is the clone published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989).
Proper citation: RRID:Addgene_16855 Copy
Species: Mus musculus
Genetic Insert: Wnt-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the vector pCS2+
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16851 Copy
Species: Mus musculus
Genetic Insert: Nuak1
Vector Backbone Description: Vector Backbone:pCIG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23791179
Proper citation: RRID:Addgene_168507 Copy
Species: Mus musculus
Genetic Insert: gRNA library targeting mouse genes
Vector Backbone Description: Backbone Marker:Kosuke Yusa (Addgene #67974); Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35451955
Proper citation: RRID:Addgene_169082 Copy
Species: Mus musculus
Genetic Insert: RiboL1-jGCaMP8s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_169247 Copy
Species: Mus musculus
Genetic Insert: KRAB-Arid3a
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169306 Copy
Species: Mus musculus
Genetic Insert: Soma-jGCaMP8s
Vector Backbone Description: Vector Backbone:pGP-AAV-syn-jGCaMP8s-WPRE; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_169256 Copy
Species: Mus musculus
Genetic Insert: Arid3a
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169318 Copy
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