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Species: Homo sapiens
Genetic Insert: cz :: caspase-3 (p17)
Vector Backbone Description: Backbone Marker:Andrew Fire Lab; Backbone Size:3880; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283333
Comments: caspase-3 (p17) fused to the antiparallel leucine-zipper domain CZ (AQLEKKLQALEKKLAQLEWKNQALEKKLAQ). For listed sequence, 1-397bp = Pmec-18; 417-506bp = CZ; 546-587bp = intron; 609-1136bp = Caspase-3(p17).
Proper citation: RRID:Addgene_16083 Copy
Species: Homo sapiens
Genetic Insert: caspase-3 (p12) :: nz
Vector Backbone Description: Backbone Marker:Andrew Fire Lab; Backbone Size:3880; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17283333
Comments: caspase-3 (p12) fused to the antiparallel leucine-zipper domain NZ (ALKKELQANKKELAQLKWELQALKKELAQ). For listed sequence, 1-397bp = mec-18p; 410-718bp = caspase-3(p12); 746-787bp = intron; 824-916bp = NZ.
Proper citation: RRID:Addgene_16082 Copy
Species: Homo sapiens
Genetic Insert: GFP-Flag-JunB-EPEA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5365; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32119511
Proper citation: RRID:Addgene_160743 Copy
Species: Homo sapiens
Genetic Insert: shPOLE1_1
Vector Backbone Description: Backbone Marker:The RNAi Consortium; Backbone Size:7466; Vector Backbone:pLKO.1P; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33152268
Proper citation: RRID:Addgene_160762 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q148A and a second mutagenesis to yield the double mutant.
Proper citation: RRID:Addgene_158633 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). This plamid has an additional Q271A mutation, and amino acid 93 (glutamate, E) was mutated back to the reference (glutamine, Q). Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q271A and a second mutagenesis to yield the double mutant. The clones displayed consistent off-target mutations at one of two points. Two clones with different off-target mutations were digested and the good pieces ligated together so to yield the final sequence.
Proper citation: RRID:Addgene_158632 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33740397
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F. This plasmid has an additional Q148A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) on the pEN_TT 3XFLAG-MAVS plasmid.
Proper citation: RRID:Addgene_158630 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: sacA crRNA
Vector Backbone Description: Backbone Size:5952; Vector Backbone:pAD123; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Shuttle vector Bacillus subtilis / E. coli; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains an E295G mutation in rep60. This mutation is not expected to affect plasmid function.
Proper citation: RRID:Addgene_158649 Copy
Species: Simian virus 40
Genetic Insert: SV40Tag
Vector Backbone Description: Backbone Marker:Addgene #13425; Vector Backbone:pLKO.1 neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Proper citation: RRID:Addgene_158646 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: Cpf1, ganA crRNA
Vector Backbone Description: Backbone Size:7956; Vector Backbone:pHT01; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Shuttle vector Bacillus subtilis / E. coli; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_158647 Copy
Species: Other
Genetic Insert: CVB3
Vector Backbone Description: Backbone Marker:Invitrogen/Addgene #2092; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: There is an internal EcoRI site in the CVB3 sequence.
Proper citation: RRID:Addgene_158645 Copy
Species: Homo sapiens
Genetic Insert: OAS2
Vector Backbone Description: Backbone Marker:Addgene #25896; Vector Backbone:pLX302; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Proper citation: RRID:Addgene_158642 Copy
Species: Homo sapiens
Genetic Insert: Mx1
Vector Backbone Description: Backbone Marker:Addgene #25896; Vector Backbone:pLX302; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33740397
Comments: Addgene NGS identified F660S variant in MX1 ORF, depositing lab confirmed this variant present in the original version of plasmid used in associated publication.
Proper citation: RRID:Addgene_158640 Copy
Species: Escherichia coli
Genetic Insert: 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
Vector Backbone Description: Vector Backbone:(na) pTol2mini?; Vector Types:Tol2-based transgenic expression in zebrafish/other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35132245
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.22.111427v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158653 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: sacA crRNA
Vector Backbone Description: Backbone Size:6499; Vector Backbone:pBSG03; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Shuttle vector Bacillus subtilis / E. coli; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_158650 Copy
Species: Homo sapiens
Genetic Insert: Tet-OsTIR1(F74G) AAVS1
Vector Backbone Description: Vector Backbone:pMK381; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_158664 Copy
Species: Homo sapiens
Genetic Insert: Tet-OsTIR1(WT) AAVS1
Vector Backbone Description: Vector Backbone:pMK364; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_158663 Copy
Vector Backbone Description: Vector Backbone:pMT; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606977
Comments: For questions regarding use of this plasmid, please contact David Root.
Please visit https://www.biorxiv.org/content/10.1101/2020.05.17.100818v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158579 Copy
Species: Synthetic
Genetic Insert: SomaGCaMP7
Vector Backbone Description: Backbone Size:4576; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32592656
Proper citation: RRID:Addgene_158759 Copy
Species: Synthetic
Genetic Insert: SomaGCaMP6f2
Vector Backbone Description: Backbone Size:4735; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32592656
Proper citation: RRID:Addgene_158757 Copy
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