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Species: Homo sapiens
Genetic Insert: Mek1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2200; Vector Backbone:pENTR1A; Vector Types:Subclone vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15342384
Comments: Constitutive active protein has the following aa sequence deleted - ALQKKLEELELDEQQRKRLE, residues 31 to 51.
Proper citation: RRID:Addgene_21207 Copy
Species: Homo sapiens
Genetic Insert: Mek2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2200; Vector Backbone:pENTR1A; Vector Types:Subclone vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15342384
Comments: Constitutive active protein has the following aa sequence deleted - EQQKKRLE, residues 48 to 55.
Proper citation: RRID:Addgene_21211 Copy
Species: Caenorhabditis elegans
Genetic Insert: cye-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21392 Copy
Species: Caenorhabditis elegans
Genetic Insert: L3787 promoter::GFP in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21377 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 promoter (no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21385 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 promoter (short, no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21384 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21389 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-56 promoter in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21383 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-3 prom in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21382 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 M1M2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21381 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
Proper citation: RRID:Addgene_21595 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21511 Copy
Species: Homo sapiens
Genetic Insert: ALIX
Vector Backbone Description: Backbone Size:4700; Vector Backbone:mCherry; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18948538
Proper citation: RRID:Addgene_21504 Copy
Species: Homo sapiens
Genetic Insert: MAP4K3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19587239
Proper citation: RRID:Addgene_21733 Copy
Species: Homo sapiens
Genetic Insert: hDlg/SAP97 PDZ2 domain
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17713926
Comments: There is a second His tag that is C-terminal on the vector after the first His tag.It is however not in frame with the insert and other His tag.
Proper citation: RRID:Addgene_21692 Copy
Species: Human papillomavirus type 16
Genetic Insert: Protein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19244625
Comments: The whole E6 gene was cloned into the vector, however a stop codon was introduced at amino acid 75, creating the truncated form.
Proper citation: RRID:Addgene_21699 Copy
Species: Homo sapiens
Genetic Insert: MAP4K3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19587239
Proper citation: RRID:Addgene_21732 Copy
Species: Human papillomavirus type 16
Genetic Insert: Protein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19244625
Proper citation: RRID:Addgene_21698 Copy
Species: Human papillomavirus type 16
Genetic Insert: protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19244625
Proper citation: RRID:Addgene_21696 Copy
Genetic Insert: ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17073440
Proper citation: RRID:Addgene_21766 Copy
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