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Species: Other
Genetic Insert: CYP119
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24924478
Comments: We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).
Proper citation: RRID:Addgene_66136 Copy
Species: Other
Genetic Insert: CYP119
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24924478
Comments: We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).
Proper citation: RRID:Addgene_66137 Copy
Species: Other
Genetic Insert: CYP119
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24924478
Comments: We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).
Proper citation: RRID:Addgene_66134 Copy
Species: Other
Genetic Insert: CYP119
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24924478
Comments: We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).
Proper citation: RRID:Addgene_66135 Copy
Species: Other
Genetic Insert: STh
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5996; Vector Backbone:pBR325; Vector Types:cloning vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:3284902
Comments: est gene probe (216 bp) cloned on EcoRI linkers into pBR325. STh = Heat stable enterotoxin gene (human) from ETEC E. coli 153837-2
Proper citation: RRID:Addgene_66148 Copy
Species: Other
Genetic Insert: perA
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pACYC184; Vector Types:cloning vector; Bacterial Resistance:Tetracycline
Comments: E coli E2348/69 PerA plasmid regulator. perA subclone from pMAR2. ~ 5 kb EcoRI fragment of pMAR2 (GenBank accession number FM180569.1) containing perA, perB and perC cloned into the EcoRI site of pACYC184.
Proper citation: RRID:Addgene_66153 Copy
Species: Other
Genetic Insert: espB
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3461; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11956862
Comments: 6XHis N-terminal tagged espB pQE30 (Qiagen). Must be grown in strain that carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome (eg XL1-Blue).
Proper citation: RRID:Addgene_66156 Copy
Species: Other
Genetic Insert: ace
Vector Backbone Description: Backbone Marker:Kaper lab, Addgene plasmid 63737; Vector Backbone:pCVD315; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8389476
Comments: Cloned ace gene from El tor Vibrio cholerae E7946 on a 2.9 Kb EcoRV fragment cloned into pCVD315. Also contains 391 bp of zot.
Proper citation: RRID:Addgene_66163 Copy
Species: Other
Genetic Insert: nag-ST
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Comments: pBR322::NAG-ST (11kb Bg1II insert) Cloned heat-stable enterotoxin from parent strain Vibrio cholera NRT36S.
Proper citation: RRID:Addgene_66161 Copy
Species: Other
Genetic Insert: tdh-like
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2323548
Comments: A 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned from the chromosome into the HindIII site of pBR322.
Proper citation: RRID:Addgene_66160 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029109. A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vector to produce the CRISPR/Cas9 binary vector. This can be used for inserting sgRNA cassette.
Please note that an IS5 transposase insertion sequence was found during Addgene's quality control process. The depositing lab noted that the presence of IS5 should not affect plasmid function.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66187 Copy
Species: Other
Genetic Insert: first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28881081
Proper citation: RRID:Addgene_67277 Copy
Species: Other
Genetic Insert: Bleomycin drug selection marker
Vector Backbone Description: Vector Backbone:pSP72; Vector Types:Leishmania Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199327
Comments: This vector can be used as the PCR template to generate the ble expression cassette donor for gene disruption with CRISPR-CAS9
Proper citation: RRID:Addgene_63561 Copy
Species: Other
Genetic Insert: ehxA
Vector Backbone Description: Vector Backbone:pBR325; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:3298451
Comments: Also reference Schmidt et al. I&I 63:1055-1061
Proper citation: RRID:Addgene_63754 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l. LEU2 1563..2592
loxP 2609..2642
Consensus Ty3 3' 2782..2982
pUC ori 3763..4406
AmpR 4509..5367
Consensus Ty3 5' 5811..6044
T ADH1 6071..6265
P TEF1 6284..6703
GFP 6713..7429
Proper citation: RRID:Addgene_63658 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. URA3 477..1277
P K.l. URA3 1278..1776
loxP 1793..1826
Consensus Ty4 3' 1981..2166
pUC ori 2964..3607
AmpR 3710..4568
Consensus Ty4 5' 5049..5233
T ADH1 5278..5472
P TEF1 5491..5910
GFP 5920..6636
Proper citation: RRID:Addgene_63654 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l. LEU2 1563..2592
loxP 2609..2642
Consensus TY2 3' 2782..2968
pUC ori 3755..4398
AmpR 4501..5359
Consensus TY2 5' 5803..6031
T ADH1 6058..6252
P TEF1 6271..6690
GFP 6700..7416
Proper citation: RRID:Addgene_63657 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. URA3 477..1277
P Kl. URA3 1278..1776
loxP 1793..1826
Consensus Ty1Cons1 3' 1966..2173
pUC ori 2946..3589
AmpR 3692..4550
Consensus Ty1Cons1 5' 4994..5227
T ADH1 5254..5448
P TEF1 5467..5886
GFP 5896..6612
Proper citation: RRID:Addgene_63650 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K. l. URA3 477..1277
P K. l. URA3 1278..1776
loxP 1793..1826
Consensus Ty1Cons2 3' 1966..2171
pUC ori 2945..3588
AmpR 3691..4549
Consensus Ty1Cons2 5' 4993..5224
T ADH1 5251..5445
P TEF1 5464..5883
GFP 5893..6609
Proper citation: RRID:Addgene_63651 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. URA3 477..1277
P K.l. URA3 1278..1776
loxP 1793..1826
Consensus Ty3 3' 1966..2166
pUC ori 2947..3590
AmpR 3693..4551
Consensus Ty3 5' 4995..5228
T ADH1 5255..5449
P TEF1 5468..5887
GFP 5897..6613
Proper citation: RRID:Addgene_63653 Copy
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