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Species: Rhodopseudomonas palustris
Genetic Insert: iRFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21765402
Comments: piRFP was derived from pEGFP-C1, whereby iRFP was inserted in place of EGFP. The iRFP in this plasmid has a stop codon at its end making it unsuitable for generating fusions. This plasmid can be used for cytoplasmic expression of iRFP or as a source of the iRFP gene.
For additional protocols on using iRFP in other tissue types please review:
Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451.
PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22213541/
For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/
Proper citation: RRID:Addgene_31857 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #21907; Backbone Size:8141; Vector Backbone:pSicoR-mCH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20498046
Comments: The pSicoR-Ef1α-mCh-Puro lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α and adding an in-frame T2A-puromycin-resistance gene cassette following mCherry. A single RNA molecule is generated for mCherry-T2A-puromycin and two polypeptides are produced by the T2A ribosomal skip motif. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.
Proper citation: RRID:Addgene_31845 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #21907; Backbone Size:7484; Vector Backbone:pSicoR-mCH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20498046
Comments: The pSicoR-Ef1α-mCh lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α.
The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.
Proper citation: RRID:Addgene_31847 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21147110
Proper citation: RRID:Addgene_31790 Copy
Species: Mus musculus
Genetic Insert: TAZ
Vector Backbone Description: Backbone Marker:Drs. S Lessnick and T. Golub (Dana-Farber Cancer Institute); Vector Backbone:pSRP; Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16099986
Proper citation: RRID:Addgene_31795 Copy
Vector Backbone Description: Backbone Size:3216; Vector Backbone:pT3TS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9891358
Proper citation: RRID:Addgene_31830 Copy
Genetic Insert: VSV genome
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21825165
Comments: This plasmid could potentially be used to generate BL2 virus. It is the responsibility of the recipient scientist to ensure that they are in compliance with their institutions’ biosafety requirements.
Proper citation: RRID:Addgene_31833 Copy
Species: Rattus norvegicus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822278
Comments: Contains NMHTG linker between myc and Strep tags.
The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone.
Proper citation: RRID:Addgene_31838 Copy
Species: Rattus norvegicus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822278
Comments: Contains NMHTG linker between myc and Strep tags.
The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone.
Proper citation: RRID:Addgene_31837 Copy
Species: Homo sapiens
Genetic Insert: achaete-scute complex homolog 1
Vector Backbone Description: Backbone Marker:home made/Crabtree lab; Backbone Size:8455; Vector Backbone:N106; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21753754
Proper citation: RRID:Addgene_31781 Copy
Species: Homo sapiens
Genetic Insert: beta-catenin
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pT3-EF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17785413
Comments: This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.
Proper citation: RRID:Addgene_31785 Copy
Vector Backbone Description: Backbone Size:9119; Vector Backbone:pGBT; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552255
Comments: The plasmid contains Tol2 transposon mini ITRs.
Proper citation: RRID:Addgene_31828 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP5G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23035093
Proper citation: RRID:Addgene_31788 Copy
Genetic Insert: GFP (lacking ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
Proper citation: RRID:Addgene_31820 Copy
Species: Homo sapiens
Genetic Insert: NeuroD2
Vector Backbone Description: Backbone Marker:home made/Crabtree lab; Backbone Size:8851; Vector Backbone:N174; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21753754
Proper citation: RRID:Addgene_31822 Copy
Species: Mus musculus
Genetic Insert: MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7565789
Comments: The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic. The wild-type RSRF sequence of the -307 to -242 Nur77/NR4A1 promoter region was cloned in to the SalI site of this SmaI/BglII fragment.
Proper citation: RRID:Addgene_31818 Copy
Species: Mus musculus
Genetic Insert: Erk5
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11046135
Proper citation: RRID:Addgene_31817 Copy
Species: Mus musculus
Genetic Insert: miR-9/9* and miR-124
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11315; Vector Backbone:pLemir; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21753754
Proper citation: RRID:Addgene_31779 Copy
Species: Homo sapiens
Genetic Insert: FADD
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21183682
Proper citation: RRID:Addgene_31814 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCS2CherDest; Vector Types:Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19269286
Comments: Expression clone for generating mRNA encoding mcherry-actMEKK.
Proper citation: RRID:Addgene_31880 Copy
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