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Species: Solanum lycopersicum
Genetic Insert: Donor + GVR
Vector Backbone Description: Backbone Marker:n/a; Vector Backbone:pLSLR; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26541286
Proper citation: RRID:Addgene_70014 Copy
Species: Solanum lycopersicum
Genetic Insert: Nuclease (Cas9/sgRNA) + Donor + GVR
Vector Backbone Description: Backbone Marker:n/a; Vector Backbone:pLSLR; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26541286
Proper citation: RRID:Addgene_70018 Copy
Vector Backbone Description: Vector Backbone:pMY200; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Comments: PCR was used to amplify a fragment containing the left T-DNA border from PBht2. Two primers were designed as follows:
TRDNAS: CAAAgTTggCgTATAACATAgT, downstream of SacII site in pBht2.
TLDNAAPmeI: ATTATAgTTTAAACATTCggCgTTAATTCAgTACA, upstream of T-border 5’ end contains PmeI site in pBht2.
2. Digested PCR product with PmeI and SacII, gel extraction followed by recovery of ~400 bp fragment, and pBht2 vector was digested with PmeI and SacII, gel extracted, followed by recovery of ~6 kb fragment. CIP dephosphatase treatment, ligated with PCR product, electroporate into E. coli.
Proper citation: RRID:Addgene_69946 Copy
Species: Synthetic
Genetic Insert: pSCKtheoRaj12m
Vector Backbone Description: Backbone Size:3376; Vector Backbone:pSTC2; Vector Types:Synthetic Biology, pSC101 origin of replication; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25916845
Comments: Please check Genbank file for complete sequence annotation.
Proper citation: RRID:Addgene_69943 Copy
Species: Synthetic
Genetic Insert: TheoHHAzRaj12,cisRaj12,sfGFP
Vector Backbone Description: Backbone Size:3376; Vector Backbone:pSTC2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25916845
Comments: Please check Genbank file for complete sequence annotation
Proper citation: RRID:Addgene_69942 Copy
Species: Synthetic
Genetic Insert: TheoHHAzRaj12,cisRaj11,sfGFP
Vector Backbone Description: Backbone Size:3376; Vector Backbone:pSTC2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25916845
Comments: Please check Genbank file for complete sequence annotation.
Proper citation: RRID:Addgene_69941 Copy
Species: Homo sapiens
Genetic Insert: RC3H2:YTC020-E12:C234063
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26117); Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4Z30 for more information
Proper citation: RRID:Addgene_69957 Copy
Species: Homo sapiens
Genetic Insert: WHSC1L1:JMC055-F10:C223308
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4YZ8 for more information
Proper citation: RRID:Addgene_69955 Copy
Species: Homo sapiens
Genetic Insert: ZCWPW2:JMC01B-H12:C203050
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4Z0O for more information
Note that PDB IDs 4Z0O & 4Z0R are the same clone.
Proper citation: RRID:Addgene_69954 Copy
Species: Homo sapiens
Genetic Insert: RC3H1:YTC019-B07:C232791
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4YWQ for more information
Proper citation: RRID:Addgene_69953 Copy
Species: Homo sapiens
Genetic Insert: ORC2:JMC095-D11:C231601
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/5C8H for more information
Proper citation: RRID:Addgene_69969 Copy
Species: Homo sapiens
Genetic Insert: ubv.it.02:YTC006-E08:C225422
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26117); Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/5C7M (complex) for more information
Note the 3D structure associated with PDB ID: 5C7M was a complex generated using 5C7M (ITCH): plasmid# 69966 and 5C7M (UBC): plasmid# 69967.
Proper citation: RRID:Addgene_69967 Copy
Species: Homo sapiens
Genetic Insert: NEDD4:SDC094-F02:C29544
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/5C7J for more information
Proper citation: RRID:Addgene_69965 Copy
Species: listeria seeligeri
Genetic Insert: LseC2c2 locus with one spacer in array
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26593719
Proper citation: RRID:Addgene_70170 Copy
Species: Danio rerio
Genetic Insert: mfap4 promoter
Vector Backbone Description: Backbone Size:2765; Vector Backbone:p5E-MCS; Vector Types:Gateway 5' Element; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26445458
Comments: Please note: Addgene's quality control sequencing has identified a number of single-nucleotide discrepancies, compared to the NCBI Genbank reference sequence for the Zebrafish mfap4 promoter. The depositing laboratory is aware of these differences, and states that they are polymorphisms. This plasmid accurately reflects the construct as it was used in the associated publication.
Proper citation: RRID:Addgene_70052 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Alexeyev Lab; Backbone Size:4064; Vector Backbone:pMA2008; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27136098
Proper citation: RRID:Addgene_70110 Copy
Species: Homo sapiens
Genetic Insert: SERT
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14660642
Proper citation: RRID:Addgene_70105 Copy
Species: Homo sapiens
Genetic Insert: SERT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pECcFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20118234
Proper citation: RRID:Addgene_70104 Copy
Species: Rattus norvegicus
Genetic Insert: GAT
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12223478
Proper citation: RRID:Addgene_70107 Copy
Species: Homo sapiens
Genetic Insert: SERT
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14660642
Proper citation: RRID:Addgene_70106 Copy
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