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Species: Synthetic
Genetic Insert: ymTq2-yomCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168066 Copy
Species: Synthetic
Genetic Insert: ymScarletI-ymNeongreen
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168067 Copy
Species: Synthetic
Genetic Insert: ymTq2-tdTomato
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168068 Copy
Species: Homo sapiens
Genetic Insert: GFP-Flag-Nup62-EPEA
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5365; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33686291
Proper citation: RRID:Addgene_168101 Copy
Species: Synthetic
Genetic Insert: ymTq2-AausFP1
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168064 Copy
Species: Synthetic
Genetic Insert: ymTq2-ystagRFPt
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168070 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc-OGA(544-706)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5352; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33686291
Proper citation: RRID:Addgene_168099 Copy
Species: Homo sapiens
Genetic Insert: HA-nGFP-(EAAAK)4-OGA(544-706)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5352; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33686291
Proper citation: RRID:Addgene_168097 Copy
Species: Synthetic
Genetic Insert: HygR gene
Vector Backbone Description: Backbone Size:2648; Vector Backbone:pUC19; Vector Types:Synthetic Biology, Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31518064
Proper citation: RRID:Addgene_168129 Copy
Species: Synthetic
Genetic Insert: yomCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168087 Copy
Species: Synthetic
Genetic Insert: yotagRFPT
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168089 Copy
Species: Synthetic
Genetic Insert: cyterm-ymTq2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_168083 Copy
Species: Synthetic
Genetic Insert: LifeAct-mTq2
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168084 Copy
Species: Synthetic
Genetic Insert: LifeAct-mScarlet
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168086 Copy
Species: Synthetic
Genetic Insert: yotagRFPT
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168090 Copy
Species: Homo sapiens
Genetic Insert: NEK9
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6100; Vector Backbone:pMRX-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078910
Proper citation: RRID:Addgene_168271 Copy
Species: Homo sapiens
Genetic Insert: NEK9
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6100; Vector Backbone:pMRX-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078910
Proper citation: RRID:Addgene_168269 Copy
Species: Synthetic
Genetic Insert: HyPer7.2DAAO-NLS
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32590330
Proper citation: RRID:Addgene_168302 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-1 cDNA, tagged with a c-myc epitope at the Sty1 site of Frizzled1 (third extracellular loop), was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16819 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-1 cDNA, tagged with an HA epitope at the Sty1 site of Frizzled1 (third extracellular loop), was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The HA-tag insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16818 Copy
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