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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pET11+PCNA3-CYP119
 
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RRID:Addgene_66136 CYP119 Other Ampicillin PMID:24924478 We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091). Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 0
pET11+PCNA3-CYP119(T214V)
 
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RRID:Addgene_66137 CYP119 Other Ampicillin PMID:24924478 We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091). Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T214V 2026-08-15 01:18:24 0
pET11a+T214V
 
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RRID:Addgene_66134 CYP119 Other Ampicillin PMID:24924478 We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091). Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T214V 2026-08-15 01:18:24 0
pET11a+D77R/T214V
 
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RRID:Addgene_66135 CYP119 Other Ampicillin PMID:24924478 We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091). Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin D77R, T214V 2026-08-15 01:18:26 0
pCVD402
 
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RRID:Addgene_66148 STh Other Tetracycline PMID:3284902 est gene probe (216 bp) cloned on EcoRI linkers into pBR325. STh = Heat stable enterotoxin gene (human) from ETEC E. coli 153837-2 Backbone Marker:Amersham; Backbone Size:5996; Vector Backbone:pBR325; Vector Types:cloning vector; Bacterial Resistance:Tetracycline 2026-08-15 01:18:24 0
pCVD450
 
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RRID:Addgene_66153 perA Other Tetracycline E coli E2348/69 PerA plasmid regulator. perA subclone from pMAR2. ~ 5 kb EcoRI fragment of pMAR2 (GenBank accession number FM180569.1) containing perA, perB and perC cloned into the EcoRI site of pACYC184. Backbone Size:4300; Vector Backbone:pACYC184; Vector Types:cloning vector; Bacterial Resistance:Tetracycline 2026-08-15 01:18:24 0
pCVD469
 
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RRID:Addgene_66156 espB Other Ampicillin PMID:11956862 6XHis N-terminal tagged espB pQE30 (Qiagen). Must be grown in strain that carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome (eg XL1-Blue). Backbone Marker:Qiagen; Backbone Size:3461; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 0
pCVD630
 
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RRID:Addgene_66163 ace Other Ampicillin PMID:8389476 Cloned ace gene from El tor Vibrio cholerae E7946 on a 2.9 Kb EcoRV fragment cloned into pCVD315. Also contains 391 bp of zot. Backbone Marker:Kaper lab, Addgene plasmid 63737; Vector Backbone:pCVD315; Vector Types:cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 0
pCVD619
 
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1+ mentions
RRID:Addgene_66161 nag-ST Other Ampicillin pBR322::NAG-ST (11kb Bg1II insert) Cloned heat-stable enterotoxin from parent strain Vibrio cholera NRT36S. Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 1
pCVD531
 
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RRID:Addgene_66160 tdh-like Other Ampicillin PMID:2323548 A 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned from the chromosome into the HindIII site of pBR322. Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 0
pYLCRISPR/Cas9Pubi-H
 
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1+ mentions
RRID:Addgene_66187 Cas9 Other Kanamycin PMID:25917172 Accession # KR029109. A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vector to produce the CRISPR/Cas9 binary vector. This can be used for inserting sgRNA cassette. Please note that an IS5 transposase insertion sequence was found during Addgene's quality control process. The depositing lab noted that the presence of IS5 should not affect plasmid function. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content 2026-08-15 01:18:24 7
LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
 
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1+ mentions
RRID:Addgene_67277 first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site Other Ampicillin PMID:28881081 Backbone Size:3000; Vector Backbone:Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:36 3
pSPble
 
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1+ mentions
RRID:Addgene_63561 Bleomycin drug selection marker Other Ampicillin PMID:26199327 This vector can be used as the PCR template to generate the ble expression cassette donor for gene disruption with CRISPR-CAS9 Vector Backbone:pSP72; Vector Types:Leishmania Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:03 3
pCVD419
 
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RRID:Addgene_63754 ehxA Other Chloramphenicol and Ampicillin PMID:3298451 Also reference Schmidt et al. I&I 63:1055-1061 Vector Backbone:pBR325; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:18:05 0
pCfB2799
 
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1+ mentions
RRID:Addgene_63658 GFP Other Ampicillin PMID:26934490 Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 6..195 loxP 243..302 T K.l. URA3 303..425 Degradation tag 426..476 K.l. LEU2 477..1562 P K.l. LEU2 1563..2592 loxP 2609..2642 Consensus Ty3 3' 2782..2982 pUC ori 3763..4406 AmpR 4509..5367 Consensus Ty3 5' 5811..6044 T ADH1 6071..6265 P TEF1 6284..6703 GFP 6713..7429 Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:18:04 1
pCfB2791
 
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1+ mentions
RRID:Addgene_63654 GFP Other Ampicillin PMID:26934490 Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 6..195 loxP 243..302 T K.l. URA3 303..425 Degradation tag 426..476 K.l. URA3 477..1277 P K.l. URA3 1278..1776 loxP 1793..1826 Consensus Ty4 3' 1981..2166 pUC ori 2964..3607 AmpR 3710..4568 Consensus Ty4 5' 5049..5233 T ADH1 5278..5472 P TEF1 5491..5910 GFP 5920..6636 Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:18:04 1
pCfB2800
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_63657 GFP Other Ampicillin PMID:26934490 Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 6..195 loxP 243..302 T K.l. URA3 303..425 Degradation tag 426..476 K.l. LEU2 477..1562 P K.l. LEU2 1563..2592 loxP 2609..2642 Consensus TY2 3' 2782..2968 pUC ori 3755..4398 AmpR 4501..5359 Consensus TY2 5' 5803..6031 T ADH1 6058..6252 P TEF1 6271..6690 GFP 6700..7416 Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:18:04 1
pCfB2795
 
Resource Report
Resource Website
RRID:Addgene_63650 GFP Other Ampicillin PMID:26934490 Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 6..195 loxP 243..302 T K.l. URA3 303..425 Degradation tag 426..476 K.l. URA3 477..1277 P Kl. URA3 1278..1776 loxP 1793..1826 Consensus Ty1Cons1 3' 1966..2173 pUC ori 2946..3589 AmpR 3692..4550 Consensus Ty1Cons1 5' 4994..5227 T ADH1 5254..5448 P TEF1 5467..5886 GFP 5896..6612 Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:18:04 0
pCfB2794
 
Resource Report
Resource Website
RRID:Addgene_63651 GFP Other Ampicillin PMID:26934490 Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 6..195 loxP 243..302 T K.l. URA3 303..425 Degradation tag 426..476 K. l. URA3 477..1277 P K. l. URA3 1278..1776 loxP 1793..1826 Consensus Ty1Cons2 3' 1966..2171 pUC ori 2945..3588 AmpR 3691..4549 Consensus Ty1Cons2 5' 4993..5224 T ADH1 5251..5445 P TEF1 5464..5883 GFP 5893..6609 Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:18:04 0
pCfB2792
 
Resource Report
Resource Website
RRID:Addgene_63653 GFP Other Ampicillin PMID:26934490 Please see previous FEMS Yeast Research paper for more details: http://femsyr.oxfordjournals.org/content/14/2/238 Feature list: T CYC1 6..195 loxP 243..302 T K.l. URA3 303..425 Degradation tag 426..476 K.l. URA3 477..1277 P K.l. URA3 1278..1776 loxP 1793..1826 Consensus Ty3 3' 1966..2166 pUC ori 2947..3590 AmpR 3693..4551 Consensus Ty3 5' 4995..5228 T ADH1 5255..5449 P TEF1 5468..5887 GFP 5897..6613 Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:18:04 0

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