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Species: Solanum lycopersicum
Genetic Insert: Nuclease (Cas9/sgRNA) + Donor + GVR
Vector Backbone Description: Backbone Marker:n/a; Vector Backbone:pLSLR; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26541286
Proper citation: RRID:Addgene_70019 Copy
Species: Homo sapiens
Genetic Insert: MKLP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22580824
Comments: Note that there are two silent mutations at position 2673 and 4203. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.
Proper citation: RRID:Addgene_70154 Copy
Species: Homo sapiens
Genetic Insert: MKLP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20451386
Comments: Note that there are two silent mutations at position 2682 and 4212. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.
Proper citation: RRID:Addgene_70145 Copy
Species: Homo sapiens
Genetic Insert: MKLP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20451386
Comments: Note that there are two silent mutations at position 2682 and 4212. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.
Proper citation: RRID:Addgene_70146 Copy
Species: Homo sapiens
Genetic Insert: MKLP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20451386
Comments: Note that there are two silent mutations at position 2682 and 4212. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.
Proper citation: RRID:Addgene_70149 Copy
Species: Homo sapiens
Genetic Insert: sfCherry11-actin
Vector Backbone Description: Backbone Marker:Michael Davidson Fluorescent Protein Collection at the UCSF Nikon Imaging center; Backbone Size:6500; Vector Backbone:pEGFP-mEmerald-actin-C-18; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26988139
Proper citation: RRID:Addgene_70221 Copy
Vector Backbone Description: Backbone Size:9841; Vector Backbone:Derivative of pBR322 and pDU1; Vector Types:Bacterial shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:2830231
Comments: Multiple references:
(i) Wolk CP, Vonshak A, Kehoe P, Elhai J (1984) Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc Natl Acad Sci USA 81: 1561-1565
(ii) Schmetterer G, Wolk CP (1988) Identification of the region of cyanobacterial plasmid pDU1 necessary for replication in Anabaena sp strain M-131. Gene 62: 101-109
(iii) Wolk CP, Cai Y, Cardemil L, Flores E, Hohn B, Murry M, Schmetterer G, Schrautemeier B, Wilson R (1988) Isolation and complementation of mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on dinitrogen. J Bacteriol 170: 1239-1244
Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch E (2007) Paired cloning vectors for complementation of mutations in the cyanobacterium Anabaena sp. strain PCC 7120. Arch Microbiol 188: 551-563
Proper citation: RRID:Addgene_70253 Copy
Genetic Insert: promoter J23101-RBS B0034-EYFP-B0015
Vector Backbone Description: Backbone Marker:Registry of Biological Parts MIT; Vector Backbone:pSB3K3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814193
Proper citation: RRID:Addgene_70252 Copy
Vector Backbone Description: Backbone Size:5941; Vector Backbone:see comments; Vector Types:Conditionally lethal; Bacterial Resistance:Kanamycin
Defining Citation: PMID:2160938
Comments: sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921).
The neomycin/kanamycin determinant in pRL278 was derived from cassette C.K3 (Elhai and Wolk CP, 1988, Gene 68: 119-138).
The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984 Gene 32: 481-485) and from a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985 Gene 37: 299-304).
Additional Reference for this plasmid:
Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153
Proper citation: RRID:Addgene_70256 Copy
Vector Backbone Description: Backbone Marker:Wolk Lab, Addgene Plasmid #70253; Vector Backbone:pRL25; Vector Types:Luciferase, cyanobacteria expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:2120040
Comments: MCS: SbfI-SalI-XmaI-KpnI-SacI
Proper citation: RRID:Addgene_70263 Copy
Vector Backbone Description: Backbone Marker:Wolk Lab; Backbone Size:7432; Vector Backbone:pRL25; Vector Types:Bacterial shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17639350
Comments: MCS: BamHI-AscI-SrfI-XhoI(PspXI)-BsiWI-AatII(ZraI)-AvrII-StuI-NsiI-SacII-ApaI-ClaI-EcoRI-SacI(EcoICRI)-KpnI(Acc65I)-SalI-SbfI-NotI
Medium copy number in E. coli. The literature (e.g., Yang et al., Res. in Microbiol. [Inst. Pasteur] 164, 127-135, 2013) says that there is substantial variability in the copy number of pDU1-based plasmids in Anabaena PCC 7120 dependent on the insert in the plasmid.
Proper citation: RRID:Addgene_70694 Copy
Genetic Insert: srpR-ssrA
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26430766
Proper citation: RRID:Addgene_70727 Copy
Species: Homo sapiens
Genetic Insert: Fibrillarin
Vector Backbone Description: Vector Backbone:pTurboRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26472758
Proper citation: RRID:Addgene_70649 Copy
Species: Homo sapiens
Genetic Insert: STIMATE
Vector Backbone Description: Backbone Size:4722; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26322679
Proper citation: RRID:Addgene_70676 Copy
Species: Drosophila melanogaster
Genetic Insert: Taf7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_70853 Copy
Species: Drosophila melanogaster
Genetic Insert: l(2)37Cd
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_70854 Copy
Species: Drosophila melanogaster
Genetic Insert: Sce
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_70840 Copy
Species: Drosophila melanogaster
Genetic Insert: CG12863
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_70842 Copy
Species: Drosophila melanogaster
Genetic Insert: CG6907
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_70841 Copy
Species: Drosophila melanogaster
Genetic Insert: CG33230
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_70845 Copy
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