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Genetic Insert: 6xHistidine_TagRFP-T_A1aY1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 12298 cloned with 6x-His-TagRFP-T_A1aY1 gene (Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80. ); Backbone Size:6074; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32886100
Proper citation: RRID:Addgene_158754 Copy
Genetic Insert: TagRFP-T_A1aY1
Vector Backbone Description: Backbone Marker:Original pTRIP vector from Nicolas Manel; Backbone Size:11628; Vector Backbone:pTRIP-CAG vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32886100
Proper citation: RRID:Addgene_158751 Copy
Species: Other
Genetic Insert: CasPhi-3
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32675376
Proper citation: RRID:Addgene_158802 Copy
Species: Synthetic
Genetic Insert: SARS2-Spike_del21_D614G
Vector Backbone Description: Backbone Size:4552; Vector Backbone:HDM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32384820
Proper citation: RRID:Addgene_158762 Copy
Species: Mus musculus
Genetic Insert: GCaMP6m-RPL10a
Vector Backbone Description: Vector Backbone:Addgene #50457; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158777 Copy
Species: Other
Genetic Insert: eforCP (for crRNA cloning)
Vector Backbone Description: Vector Backbone:Pacyc184; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28646112
Proper citation: RRID:Addgene_158711 Copy
Genetic Insert: Hemagglutinin A/TX/50/12 H3
Vector Backbone Description: Backbone Marker:-; Backbone Size:5400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:32710576
Proper citation: RRID:Addgene_158739 Copy
Species: Synthetic
Genetic Insert: mitoTALEN left and right to target orf125 (orf138)
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31285556
Comments: Tandem expression vector for right and left mitoTALENs to cut kosena-type CMS gene orf125 (also cut Ogura CMS gene orf138)
Proper citation: RRID:Addgene_158737 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E3 compatible to be used as the final insertion vectors to make right TALEN, instead of ptCMV-136/63-VR-(NI), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158734 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E3 compatible to be used as the final insertion vectors to make right TALEN, instead of ptCMV-136/63-VR-(NN), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158735 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E3 compatible to be used as the final insertion vectors to make right TALEN, instead of ptCMV-136/63-VR-(HD), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158732 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E1 compatible to be used as the final insertion vectors to make left TALEN, instead of ptCMV-136/63-VR-(NI), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158730 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E1 compatible to be used as the final insertion vectors to make left TALEN, instead of ptCMV-136/63-VR-(NN), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158731 Copy
Species: Homo sapiens
Genetic Insert: Rheb
Vector Backbone Description: Backbone Size:4708; Vector Backbone:pRK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12906785
Proper citation: RRID:Addgene_15888 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Size:2300; Vector Backbone:bb118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32748785
Comments: GCaMP6f was engineered in the paper above, then codon optimized for E. coli on a G-block and cloned into our bb100 backbone to facilitate constitutive expression . Please visit https://doi.org/10.1101/2020.04.23.058362 for bioRxiv preprint.
Proper citation: RRID:Addgene_158983 Copy
Species: Synthetic
Genetic Insert: Cytbow
Vector Backbone Description: Backbone Marker:Genscript inc; Backbone Size:2530; Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24507188
Proper citation: RRID:Addgene_158995 Copy
Species: Synthetic
Genetic Insert: dSaCas9-VP64
Vector Backbone Description: Vector Backbone:pX601 (Addgene #61591); Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31108320
Proper citation: RRID:Addgene_158990 Copy
Species: Homo sapiens
Genetic Insert: RhoA
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10087266
Comments: There is an additional F25N mutation in the sequence. This change greatly facilitated expression of the protein in E.coli. The only known effect of this mutation may be negatively affect the transduction of RhoA signaling to DGK1 kinase. Otherwise the depositing lab have never found any discrepancies with other published work on activity or protein interactions.
Proper citation: RRID:Addgene_15899 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: ORF is at 258-2237.
The Addgene Quality Control sequencing results have found silent nucleotide changes in the depositor GSK3 beta sequence as well as an amino acid change. The verified sequence matches PBD Sequence ID: 4IQ6_A and encodes an isoform of human GSK3 beta that should function as WT, please review the Addgene QC sequence before use.
Proper citation: RRID:Addgene_15898 Copy
Species: Homo sapiens
Genetic Insert: GSK3 alpha
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2164470
Comments: ORF is at 1189-2640.
Proper citation: RRID:Addgene_15896 Copy
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